Heavy Metals in Edible Green Vegetables Grown Along the Sites of the Zanjanrood River in Zanjan, Iran
Gh. R. Jahed Khaniki ,
M. Nurani ,
M. Mehrasbi ,
The study investigated the levels of five different heavy metals (Cd, Pb, Zn, Cr and As) in various vegetables including roots and leaves of radish (Raphanus sativus L.), leek (Allium ampeloprasum L.), sweet basil (Ocimum basilicum L.) and parsley (Petroselinum crispum) cultivated along the bank of river passing through the city of Zanjan. The contributions of the vegetable to the daily intake of the heavy metals from the vegetables were determined. Atomic absorption spectrometry was used to estimate and evaluate the levels of these metals in the vegetables. The results of this survey showed the following ranges (mg kgG1): 3.89-32.94, 3.15-27.68, 43.61-223.10, non-detectable and non-detectable for lead, cadmium, zinc, chromium and arsenic, respectively. Some vegetables contaminated high levels beyond the levels given by FAO and WHO for human consumption. When the mean levels of Lead and Cadmium (10.65 and 9.22 mg kgG1) were taken into account the daily intake contribution of the metals was found to be 2.32 and 2 mg for Lead and Cadmium. Increase in vegetable consumption by community the situation could worse in the future.
Vegetables constitute essential components of the diet by contributing protein,
vitamins, iron, calcium and other nutrients which are usually in short supply
(Thompson and Kelly, 1990). They also act as buffering agents for acid substances
obtained during the digestion process. However, these plants contain both essential
and toxic elements over a wide range of concentrations. Chronic low level intakes
of heavy metals have damaging effects on human beings and other animals, since
there is no good mechanism for their elimination. Metals such as lead, mercury,
cadmium and copper are cumulative poisons. These metals cause environmental
hazards and are reported to be exceptionally toxic (Ellen et al., 1990).
Vegetables take up metals by absorbing them from contaminated soils as well
as from deposits on parts of the vegetables exposed to the air from polluted
environments (Zurera et al., 1989). A number of factors influence the
concentration of heavy metals on and within plants. These factors include climate,
atmospheric deposition, the nature of soil on which the plants is grown, application
of fertilizers and irrigation with wastewater (Anyanwu et al., 2004;
Khairiah et al., 2004; Itanna, 2002; Madyiwa et al., 2002; Denkota
and Schmidt, 2000; Frost and Ketchum, 2000). The water of rivers can be polluted
by heavy metals include Pb, Cu, Zn, Fe, Cr, Cd and Hg. The major sources of
heavy metals are industrial effluents and indiscriminate disposal of domestic
or sewage drainage directed to the rivers untreated or partially treated. Heavy
metals, in general, are not biodegradable, have long biological half-lives and
have the potential for accumulation in the different body organs leading to
unwanted side effects (Jarup, 2003; Sathawara et al., 2004). Lead and
cadmium are among the most abundant heavy metals and are particularly toxic.
The excessive content of these metals in food is associated with etiology of a number of diseases, especially with cardiovascular, kidney, nervous as well as bone diseases (WHO, 1992 and 1995; Steenland and Boffetta, 2000; Jarup, 2003). In suburban areas, the use of industrial or municipal wastewater is common practice in many parts of the world (Sharma et al., 2007) including Zanjan City. Zanjan City is located in the north-west of Iran. In Iran, Zanjan City in particular, it has been a common practice to cultivate vegetables along the banks of the rivers passing through the city. Due to the population increasing as well as rapid development of agriculture and industry and lack of strict legislation and regulation, heavy metals, such as Cu, Zn, Pb, Cd, Cr, As and Hg are emitted into environment in large quantities through atmospheric deposition solid waste emissions and wastewater irrigation. It is therefore anticipated that plants grown along the banks of the river Zanjanrood is not free from heavy metal pollution and may likely result in adverse health effects to the population of Zanjan City. The aim of the this study is determination of heavy metals in selected green vegetables grown along the banks of the Zanjanrood river and to estimate the daily intake of these metals.
MATERIALS AND METHODS
Sample collection: Vegetable samples of roots and leaves of radish (Raphanus sativus L.), leek (Allium ampeloprasum L.), sweet basil (Ocimum basilicum L.) and parsley (Petroselinum crispum) were randomly collected along the cultivated banks of the river zanjanrood (Fig. 1). The samples were collected from these growing areas over a period of four months during the dry season (July-October) of the year 2004. A total of 40 samples of five vegetables collected. All samples were collected and stored in polythene bags according to their type and brought to the laboratory for preparation and treatment.
Sample preparation and treatment: For lead, cadmium, chrome, arsenic
and zinc analysis vegetable samples were washed with distilled water to eliminate
air-borne pollutants. The leafy stalks were removed from all samples and these
were sliced and dried on a sheet of paper to eliminate excess moisture. Once
dried, each sample was weighed and oven-dried at 60°C to constant weight.
Each oven-dried sample was ground in a mortar until it could pass through a
60 mesh sieve. The samples stored in a clean, dry and high density polyethylene
bottles, 100 mL capacity, with screw caps.
|| Map of the studied area
Bottles were pre-washed with nitric acid, rinsed with de-ionized water, dried
and tested for contamination by leaching with 5% nitric acid. The bottles contained
no metal liners that can contaminate the samples.
Sample extraction: Samples were precisely weighed (2 g each) and ground in mortar followed by wet digestion with HNO3:HClO4 (2:1) in the conical flask for 2-3 h on a sand bath. Some 10 mL of HCl was added to solute inorganic and oxides salt. Digested samples were filtered with 0.45 μm pore size cellulose nitrate membrane filter paper (Millipore) and made up to 100 mL with distilled water and bottles were stored until the flame atomic absorption spectrophotometry was performed.
Sample analyzation: The samples were analyzed by atomic absorption spectrophotometer (ALPHA 4, Chem Tech Analytical Co. England) using a nitrous oxide-acetylene flame for As and an air-acetylene flame for four heavy metals Pb, Cd, Cr and Zn, using at least two standard solutions for each metal. The certified standard reference material (Alpha-Line, Chem Tech Analytical, Ltd, England) was used to check the accuracy and the analytical values were within the range of certified values. All the recoveries of the metals studied were over 95%. All the concentration of the metals is expressed in mg kg-1 in dry weight.
Statistical analysis: All statistical analyses were performed using
the Microsoft EXCEL (version 2003). Analysis of Kruskal-Wallis test were employed
to examine statistical significant of differences in the mean concentration
of metals between group of families of vegetables using SPSS, version 11.5.
A probability level of p<0.05 was considered statistically significant.
RESULTS AND DISCUSSION
The heavy metal concentrations were determined based on vegetable dry weight
(Table 1). The results showed that the levels of Pb in all
commodities were between 3.89 mg kg-1 in parsley and 32.94 mg kg-1
in leaves of radish, respectively. Cd contents varied from 3.15 mg kg-1
in leek to 27.68 mg kg-1 in sweet basil. The levels of Pb and Cd
in all vegetables were higher than recommended maximum residues of these heavy
metals in edible vegetables by of Codex Alimentarius Commission (Table
1). The contents of some trace elements such as As and Cr were no detectable
amount in all analyzed vegetable samples. The highest concentrations of Pb were
in radish leaves among selected vegetables. Further, the highest concentration
of Cd and Zn were observed in sweet basil and radish leaves. Leafy vegetables
appear to contain high levels of Pb and Cd. This trend is similar to those reported
in Iran (Samarghandi et al., 2000) Egypt (Dogheim et al., 2004;
Radwan and Salama, 2006), Pakistan (Parveen et al., 2003), Greece (Karavoltsos
et al., 2002; Denkota and Schmidt, 2000) Tanzania (Bahemuka and Mubofu,
1999) Ethiopia (Rahlenbeck et al., 1999) USA (Pennington et al.,
1995). The results of present study showed that the levels of Pb and Cd are
higher than that were found in literature. Bahemuka and Mubofu (1999) determined
four heavy metals includes cadmium, copper, lead and zinc in some green vegetables
cultivated along the sites of the Sinza and Msimbazi rivers in Dares Salaam,
Tanzania by Atomic absorption spectrophotometry. They reported that the contributions
of the heavy metals from the vegetables were the following ranges (100 mg g-1):
0.01±0.06, 0.25±1.60, 0.19±0.66 and 1.48±4.93 for
cadmium, copper, lead and zinc, respectively. Some vegetables contained high
levels beyond the permissible levels given by FAO and WHO for human consumption.
Pless-Mulloli et al. (2001) determined the contamination of vegetables
with the heavy metals includes copper, lead and zinc. The examined vegetables
were cabbage, carrot, turnip, courgette, beetroot, potato, parsnip and swede.
The average level of all heavy metals in all types of vegetables was low. They
were also under the recommended guideline values for commercially grown foods
and lower or well in line with levels in a UK National Total Diet Study (FSA,
Samarghandi et al. (2000) determined the concentrations of Pb, Ni, Cd and Cr in vegetables which are irrigated with polluted water in Hamadan City, Iran.
|| Concentrations of heavy metals in vegetables cultivated along
the sites of Zanjanrood river, in terms of mg kg-1 dry weight
|ND = Not detected, Levels were below the detection limit,
Values in the parenthesizes are minimum and maximum concentration of each
element, aSource: Codex Alimentarius Commission (2001)
They reported that Pb concentration in vegetables was more than the permissible
limitation for human foods. The concentrations of other heavy metals were lower
than permissible limitation for human foods. In present study, the levels of
Cd and Pb are 9.22 and 10.65 mg kg-1 and they are 35 and 46 times
above the permissible levels set by FAO and WHO for human consumption, respectively
(Fig. 2). However, other amounts were within the acceptable
levels. The variations of metal contents in these vegetables can be related
to the physical and chemical nature of soil and absorption capacity of each
metal by plant. They are altered by innumerable environmental and human factors
and nature of the plant (Zurera et al., 1989; Sharma et al., 2007).
In present study, significant differences were found in Pb concentrations between
selected edible vegetables grown along the banks of Zanjanrood river (p = 0.00,
Kruskal-Wallis test). The results showed that there are no significant differences
among the Cd concentrations of vegetables. In a research, Hibber et al.
(1984) reported that the concentrations of cadmium and lead is below the range
(0.09 to 0.26 mg 100 g-1 of cadmium, and 1.1 to 1.7 mg 100 g-1
of lead) for vegetables grown in Metropolitan Boston and Washington DC (Hibber
et al., 1984). Further, the cadmium and lead levels reported in this
study were lower than those reported for vegetables in Nigeria (Ndiokwere, 1984).
The National Nutrition and Food Research Institute of Iran have estimated that
the average consumption of edible vegetables is 218 g person-1 day-1.
Present study showed that the mean levels of Pb and Cd is 10.65 and 9.22 mg
kg-1, respectively. Therefore, the dietary intake of Cd and Pb can
be 2.32 and 2 mg day-1, respectively. Other studies from various
countries have reported that the dietary intake for lead in adult is between
54 μg day-1 (Debeca et al., 1987) and 412 μg day-1
(Dick et al., 1987). Also, the dietary intake for cadmium is between
10 and 30 mg day-1 (Reilly, 1991).
||Average concentration of heavy metals in edible green vegetables
It can be concluded that estimation of daily intake for lead in present study
is above those reported from other countries, whereas the estimation for cadmium
is below the estimations. Also, the estimated daily intake for the Pb an Cd
in this study are above that those reported by FAO/WHO (30 μg day-1
and 4.67 μg day-1) who has set a limit for heavy metal intake
based on body weight for an average adult (60 kg b.wt.) (Codex Alimentarius
Commission, 2001). Bahemuka and Mubofu (1999) reported that the daily intake
contribution of heavy metals in vegetables in Dares Salaam, Tanzania was found
to be 21.60, 858.60, 426.60 and 3.65 mg for cadmium, copper, lead and zinc,
Zinc is an essential element for plants and animals, but the slight increase in its levels may interfere with physiological processes. Sufficient Zn is essential to neutralize the toxic effects of Cd. In this study, the highest and the lowest of zinc were detected 223.10 mg kg-1 and 43.61 mg kg-1 in leaves and the roots of radish, respectively. The results showed that there is a significant variation in the concentration of Zn among the studied vegetables (p = 0.005, Kruskal-Wallis).
Comparison of the levels of heavy metals in the studied vegetables indicated that the permissible levels must be done for a safe food. The result clearly showed a divergence from the permissible levels by FAO and WHO. High contents of lead and cadmium were found in radish and sweet basil leaves, respectively. Further, there is a potential health hazard posed by the residues of heavy metals in edible vegetables when the high quantity of vegetables is consumed by people. A regulatory law implementation in vegetables products and long term planning is required to do vegetables safety. In addition, there are other items such as training of personnel or current good manufacturing practices and regular monitoring of heavy metals from effluents, sewage and edible vegetables. Monitoring can accompany by regular reevaluation of the acceptable levels must continue, but with the realization that some heavy metals will probably always be found in very low quantities and they are considered to be unavoidable contaminants.
The authors gratefully acknowledge that the present research is an out put of a collaborative research project entitled Assessment of heavy metal content of vegetables grown in the banks of Zanjanrood River which is funded by Management and Planning Organization of Zanjan. We wish to thank the Deputy of Research of Zanjan University of Medical Sciences for its administrative support and Institute for Advanced Studies in Basic Sciences, Gavazang-Zanjan for technical assistance.
Anyanwu, E.C., E.J.E. Ijeoma Kanu and M.A. Saleh, 2004. Bioavailable of lead concentration in vegetable plants grown in soil from a reclaimed industrial site: Health implications. Int. J. Food Saf., 6: 31-34.
Direct Link |
Bahemuka, T.E. and E.B. Mubofu, 1999. Heavy metals in edible green vegetables grown along the sites of the Sinza and Msimbazi rivers in Dares Salaam, Tanzania. Food Chem., 66: 63-66.
Direct Link |
Codex Alimentarius Commission, 2001. Food additives and contaminants joint codex alimentarius commission. FAO/WHO Food Standards Programme, ALINORM 01/12A, pp: 1-289.
Debeca, R.W., A.D. Mckenzie and G.M.A. Lacroix, 1987. Dietary intakes of lead, cadmium, arsenic and fluoride by Canadian adults, 24 h duplicate diet study. Food Addit. Contam., 4: 89-102.
Denkota, B. and G.H. Schmidt, 2000. Accumulation of heavy metals in food plants and grasshoppers from the Taigetos Mountains. Greece Agric. Ecosyst. Environ., 78: 85-91.
Dick, G.L., J.T. Hughes, J.W. Mitchell and F. David, 1987. Survey of trace elements and pesticides in New Zealand. J. Sci., 21: 57-69.
Dogheim, S.M., M.M. El-Ashraf, S.A.G. Alla, M.A. Khorshid and S.M. Fahmy, 2004. Pesticides and heavy metals levels in Egyptian leafy vegetables and some aromatic medicinal plants. Food Addit. Contam., 21: 323-330.
Direct Link |
Ellen, G., J.W. van Loon and K. Tolsma, 1990. Heavy metals in vegetables grown in the Netherlands and in domestic and imported fruits. Zeitschrift Lebensmittel Untersuchung Forschung, 190: 34-39.
CrossRef | Direct Link |
FSA., 1999. Mean concentrations of aluminium (Al), arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), selenium (Se), tin (Sn) and zinc (Zn) in the 20 food groups of the 1997 UK total diet study. Food Standards Agency, Food Surveillance Information Sheet Number 191: MAFF Archive. http:www.foodstandards.gov.uk/maff/archive/food/infsheet/1999/no191/table2.htm.
Frost, H.L. and L.H. Ketchum, 2000. Trace metal concentration in durum wheat from application of sewage sludge and commercial fertilizer. Adv. Environ. Res., 4: 347-355.
Hibber, C.R., S.S. Hagar and C.P. Mazza, 1984. Comparison of cadmium and lead contents of vegetable crops grown in urban and suburban gardens. Environ. Pollut., 7: 7-176.
Itanna, F., 2002. Metals in leafy vegetables grown in Addis Ababa and toxicological implications. Ethiop J. Health Dev., 16: 295-302.
Direct Link |
Jarup, L., 2003. Hazards of heavy metal contamination. Br. Med. Bull., 68: 167-182.
Direct Link |
Karavoltsos, S., A. Sakellari, M. Dimopoulos, M. Dasenakis and M. Scoullos, 2002. Cadmium content in foodstuffs from the Greek market. Food Addit. Contam., 19: 954-962.
Direct Link |
Khairiah, J., M.K. Zalifah, Y.H. Yin and A. Aminah, 2004. The uptake of heavy metals by fruit type vegetables grown in selected agricultural areas. Pak. J. Biol. Sci., 7: 1438-1442.
CrossRef | Direct Link |
Madyiwa, S., M. Chimbari, J. Nyamangara and C. Bangira, 2002. Cumulative effects of sewage sludge and effluent mixture application on soil properties of a sandy soil under a mixture of star and kikuyu in Zimbabwe. Phys. Chem. Earth, 27: 747-753.
Ndiokwere, C.L., 1984. A study of heavy metal pollution from motor vehicle emissions and its effect on roadside soil, vegetation and crops in Nigeria. Environ. Pollut. Ser. B: Chem. Phys., 7: 35-42.
CrossRef | Direct Link |
Parveen, Z., M.I. Khuhro and N. Rafiq, 2003. Market basket survey for lead, cadmium, copper, chromium, nickel and zinc in fruits and vegetables. Bull. Environ. Contam. Toxicol., 71: 1260-1264.
PubMed | Direct Link |
Pennington, J., S. Schoen, G. Salmon, B. Young, R. John and R. Mart, 1995. Composition of core foods of the USA food supply 1982-1991. II. Calcium, magnesium, iron and zinc. J. Food Compos. Anal., 8: 129-169.
Direct Link |
Pless-Mulloli, T., O. Papke and B. Schilling, 2001. PCCD/PCDF and heavy metals in vegetable samples from newcastle allotments: Assessment of the role of ash from the byker incinerator. Byker ash steering group report. University of Newcastle, Ergo Forschungsgesellschaft mbH (Ergo Research Laboratory), Hamburg, Germany.
Radwan, M.A. and A.K. Salama, 2006. Market basket survey for some heavy metals in Egyptian fruits and vegetables. Food Chem. Toxicol., 44: 1273-1278.
Rahlenbeck, S.I., A. Burberg and R.D. Zimmermann, 1999. Lead and cadmium in Ethiopian vegetables. Bull. Environ. Contam. Toxicol., 62: 30-33.
Direct Link |
Reilly, C., 1991. Metal Contamination of Foods. 2nd Edn., Elsevier Applied Science, London.
Samarghandi, M., M. Karimpoor and Gh. H. Sadri, 2000. Determination of heavy metals in Irrigated vegetables with polluted water in hamadan city. J. Man Environ., 5: 24-31.
Sathawara, N.G., D.J. Parikh and Y.K. Agarwal, 2004. Essential heavy metals in environmental samples from Western India. Bull. Environ. Contam. Toxicol., 73: 756-761.
CrossRef | PubMed | Direct Link |
Sharma, R.K., M. Agrawal and Marshall, 2007. Heavy metal contamination of soil and vegetables in suburban areas of Varanasi, India. Ecotoxicol. Environ. Safety, 66: 258-266.
Direct Link |
Steenland, K. and P. Boffetta, 2000. Lead and cancer in humans: Where are we now? Am. J. Ind. Med., 38: 295-299.
Direct Link |
Thompson, H.C. and W.C. Kelly, 1990. Vegetable Crops. 5th Edn., McGraw Hill Publishing Company Ltd., New Delhi.
WHO, 1992. Environmental Health Criteria 134: Cadmium. World Health Organisation, Geneva.
WHO., 1995. Lead Environmental Health Criteria. Vol. 165, World Health Organization, Geneva.
Zurera-Cosano, G., R. Moreno-Rojas, J. Salmeron-Egea and R.P. Lora, 1989. Heavy metal uptake from greenhouse border soils for edible vegetables. J. Sci. Food Agric., 49: 307-314.