Biodiesel is a renewable energy source which can be produced from vegetable
oils and animal fats. Biodiesel as an alternative fuel has numerous advantages
over conventional fossil fuels such as, biodegradability, renewability, high
combustion efficiency, low sulfur and aromatic content (Ma
and Hanna, 1999) and high Cetane number and flash point as high as fossil-based
diesel (Mudge and Pereira, 1999). In addition, being
of domestic origin reduces the dependency on imported petroleum which is another
advantage of biodiesel (Mittelbach and Remschmidt, 2004).
The processes used for biodiesel production are well known. Among the available methods to produce biodiesel, transesterification is the method of choice. In this method, triglycerides which are the main components of vegetable oils, react with an alcohol (e.g., methanol or ethanol) in presence of a catalyst (e.g., alkali, Acid or enzyme) to produce Fatty Acid Alkyl Esters (FAAE) and glycerol as by-product.
Methanol is the most common alcohol used in transesterification reactions because
of its low price compared to other alcohols. The stoichiometry of the reaction
demands three moles of methanol and one mol of triglyceride to produce three
moles of fatty acid methyl ester and one mole of glycerol. This reaction consists
of three consecutive reversible reactions with intermediate formation of diglycerides
and monoglycerides as shown in Fig. 1. At the end of reaction,
glycerol is separated by settling or centrifuging.
||Three consecutive reversible reactions to produce FAAM
The glycerol obtained is further purified before using it for its traditional
applications or for new applications (Vicente et al.,
However, the produced biodiesel contains a significant quantity of glycerol and another purification stage of biodiesel is necessary before using it as an automobile fuel.
As indicated in Fig. 1, the triglyceride molecule loses three FAME molecules in a complete reaction. However, this reaction does not go to completion and the product will be contaminated with un-reacted triglycerides from the parent vegetable oil and the intermediates mono- and diglycerides (acylglycerols). These are referred to as bound glycerol. Another source of glycerol found in the final biodiesel is the remaining glycerol that has not been removed from the biodiesel during the purification step. The latter is referred to as free glycerol. The sum of the bound and the free glycerol is referred to as total glycerol.
The presence of total glycerol initiates engine corrosion and on the long term
affects human or animal health by emission of hazardous acrolein into the environment.
Moreover, the quality of the final biodiesel depends on its glycerol content.
The viscosity of the glycerol present in the mixture hinders the high-pressure
injection system of a modern diesel engine and may cause damage (Abbott
et al., 2007). Accordingly, maximum allowable amounts of free and
total glycerol are included in the biodiesel specification of most countries.
The finally produced biodiesel should fulfill EN 14214 and ASTM D6751 standard
There are several methods for removing glycerol from biodiesel including, wet
and dry washing of the biodiesel product (Berrios and Skelton,
2008), adsorption over silica (Yori et al., 2007),
using membrane reactors (Dube et al., 2007) and
the addition of lime and phosphoric acid to the biodiesel product (Chiu
et al., 2006). However, using these methods, the final production
cost is increased due to materials expenses and process complications. Moreover,
the above-mentioned methods are not environmentally benign because of a massive
discharge of wastewater.
Ionic Liquids (ILs) are composed of discrete cations and anions which are liquid
at or below 100°C. In recent years, because of ILs unusual properties, they
have attracted more attention and became interesting for many applications.
Thus, they have great potential as green solvents for industrial processes (Rogers
and Seddon, 2002). Ionic liquids have been described as designer solvents
(Freemantle, 1998) and this means that their properties
can be tuned to suit the requirements of a particular process. Properties such
as melting point, viscosity, density and hydrophobicity can be varied by simple
changes to the structure of the ions (Martyn and Kenneth,
2000). ILs do not evaporate due to their very low vapor pressures and they
have great ability to dissolve many different organic, inorganic and organometallic
materials. In addition to all these properties, they are immiscible with many
organic solvents and are highly polar.
Due to the high costs of ILs, Deep Eutectic Solvent (DES) can be utilized as
a low cost alternative. Currently, DESs are being used in research as well as
in industry because of their potential as environmentally benign solvents and
advantages such as non-toxicity, non-reactivity with water and being biodegradable
(Abbott et al., 2004). In fact, DESs represent
common solvation properties with ionic liquids. They are formed by mixing two
or more components and they have low melting point compared to their compounds.
In other words, they are created from mixtures of organic halide salts with
an organic compound which is a Hydrogen Bond Donor (HBD) able to form a hydrogen
bond with the halide ion (Liu et al., 2008).
Freezing point depression causes the liquid state of DES, whereby hydrogen-bonding
interactions between an anion and an HBD are more energetically favored relative
to the lattice energies of the pure constituents (Nkuku
and LeSuer, 2007).
Abbott et al. (2004 and 2003)
have shown that ILs created from eutectic mixture of quaternary ammonium salts
and hydrogen bond donors is an efficacious technique of producing inexpensive,
non-toxic and environmentally benign solvents systems. These deep eutectic solvents
have practical uses in some applications like electropolishing and metal oxide
processing (Abbott et al., 2005, 2006).
Moreover, they have shown that a glycerol based DES can be used successfully
as an extraction media for glycerol from biodiesel product (Abbott
et al., 2007).
Recently, Hayyan et al. (2010) showed that ionic
liquid based on a low cost quaternary ammonium salt (choline chloride) and glycerol
can be used as a solvent for extracting glycerol from palm oil-based biodiesel
in a continuous separation process. The effect of DES to biodiesel ratio and
the composition of DES on the efficiency of extraction process were investigated.
The best ratio of DES:biodiesel was (1:1) and the DES composition was (1:1,
In an attempt to improve the abovementioned work, we present two new successful deep eutectic solvents to remove all free glycerol from palm oil-based biodiesel. The ratios of DES composition and DES to biodiesel were optimized for maximum removal of total glycerol.
MATERIALS AND METHODS
Chemicals: Palm oil (FFM Sdn Bhd), methanol (CH3OH, Merck 99%), potassium hydroxide (KOH, HMGM Chemicals > 98%), choline chloride (C5H14ClNO, Merck 99%), ethylene glycol (HOCH2CH2OH, Merck 99%) and 2,2,2-trifluracetamide (C2H2F3NO, Merck 99%) were obtained from commercial sources and used in synthesis of biodiesel and DESs without further purification.
Synthesis: To synthesize biodiesel, potassium hydroxide as a catalyst
(1 wt% of palm oil) was suspended in methanol and shaken until potassium hydroxide
was dissolved in methanol to form potassium methoxide. Afterwards, potassium
methoxide was added to palm oil with 1:10 molar ratio (Cheng
et al., 2004) of palm oil to methanol in a batch reactor with 400
rpm mixing speed at 50°C for 2 h. The products were transferred to a separation
funnel and after overnight settling, biodiesel phase (upper layer) was separated
from glycerol-rich phase (lower layer).
|| Different DESs synthesized for removal of glycerol
In this study two DESs were utilized for extraction of glycerol, in the first
a combination of choline chloride (ChCl) with ethylene glycol (as an HDB) was
used whereas 2,2,2-trifluracetamide was used as the HBD in second. After drying Choline chloride under vacuum, it was mixed with ethylene glycol
in different Salt: Ethylene glycol molar ratios (1:1.75, 1:2, 1:2.25 and 1:2.5)
at 60°C and 300 rpm agitation until a homogenous transparent liquid appeared.
For the synthesis of the 2,2,2-trifluracetamide based DES, ChCl was mixed with
2,2,2-trifluracetamide in different molar ratio (1:1.5, 1:1.75, 1:2 and 1:2.5)
with mixing speed of 400 rpm at 90°C for 3 h followed by filtration of products.
Table 1 shows the compositions of the different DESs synthesized
in this study.
DESs characterization: Viscosities of the synthesized DESs were measured at 25°C using a rotary viscometer (Brookfield R/S plus Rheometer). Melting points of the DESs were determined by Mettler Toledo Differential Scanning Calorimetry (DSC).
Extraction method: To perform liquid-liquid extraction, each DES was added to the biodiesel phase separately in different molar ratios (ranging 0.75-3 molar units DES per 1 molar unit biodiesel) and then the samples were mixed for 1 h at room temperature with a shaking speed of 200 rpm using an orbital shaker. After two hours settling, the upper layers of samples were separated and analyzed for their free and total glycerol content by gas chromatography.
Gas chromatography analysis: The analysis of free and total glycerol in palm oil-based biodiesel was performed according to ASTM method of D6584 and CEN method of EN14105. The GC specifications and analysis conditions are given in Table 2. To prepare samples for GC analysis, butanetriol (internal standard#1) was used to identify free glycerol peak and then tricaprin (internal standard#2) was added to indentify the monoglycerides, diglycerides and triglycerides peaks. Also, N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) was used to improve volatility and reduce activity before injection into the GC. Finally the samples were injected into the gas chromatograph, once they were prepared.
|| GC specifications and analysis conditions
RESULTS AND DISCUSSION
DESs characterization: A quaternary ammonium based salts (choline chloride) and two different hydrogen-bond donors (ethylene glycol and 2,2,2-trifluracetamide) were selected for the synthesis of DESs in different salt to HBD molar ratios. The choline chloride was mixed with ethylene glycol as an HBD (DES1, DES2, DES3 and DES4). Figure 2 shows the melting points and viscosities of the different DESs as a function of ethylene glycol mole fraction. As this figure demonstrates, with the increase in ethylene glycol mole fraction, DESs viscosities declined gradually.
The decrease in viscosity can be attributed to the lower viscosity of ethylene glycol. Figure 2 also indicates that the minimum eutectic temperature was at 0.667 ethylene glycol mole fraction (DES2) corresponding to -66°C. In addition, this figure shows that DES1, DES2, DES3 and DES4 have very low freezing points which were congruous with physical properties of deep eutectic solvents. Low viscosity of DESs causes an increase in the mixing efficiency and using DESs that have lowest freezing point raises the amount of glycerol that can be extracted without causing the freezing point of the DES to increase above the process temperature. This in turn improves the solvent extractive capabilities.
The 2,2,2-trifluracetamide based DES was synthesized following the same procedure
described above. The different synthesized ratios are coded DES5, DES6, DES7
and DES8. Figure 3 presents freezing points and viscosities
of these DESs versus 2,2,2-trifluracetamide mole fraction.
||Freezing point and viscosity as a function of ethylene glycol
mole fraction for DES1, DES2, DES3 and DES4
||Freezing point and viscosity as a function of 2,2,2-trifluoroacetamide
mole fraction for DES5, DES6, DES7 and DES8
As can be seen the freezing points of the above DESs were all below ambient
The eutectic point for the ChCl:2,2,2-trifluracetamide DES was not clear from Fig. 3 because mixing of any ratio above 1:2.5 resulted in a white precipitate, signifying that saturation had occurred and some of the salt or HBD is not contributing to the DES, thus the ratio of the resulting DES is not the same as intended to be. Nevertheless, the minimum freezing point attained was -45C with a corresponding viscosity of 0.178 Pa.s which makes it very convenient for use as solvent for liquid-liquid extraction separation, provided that the solute has reasonable mass transfer affinity to the DES.
Extraction of glycerol: Free Glycerol was extracted from palm oil-based
biodiesel using liquid-liquid extraction with DES as a solvent.
||Gas chromatograms for biodiesel combinations: (a) before extraction
and (b) after extraction
For the extraction
process, eight molar ratios of DES to biodiesel (0.75:1, 1:1, 1.25:1, 1.5:1,
1.75:1, 2:1, 2.5:1 and 3:1) were prepared for each of the synthesized DESs (DES1,
DES8). After mixing the DES with biodiesel and leaving it for about two hours for
settling, two separate layers of biodiesel and DES were distinguished and the
DES layer was separated completely.
Gas chromatography analysis of the biodiesel before extraction indicates that free glycerol content was 0.04093 (wt.%). This is higher than free glycerol content specified by the EN 14214 and ASTM D 6721 standards (0.02 mol% and 0.020 wt.%, respectively). However, the total glycerol content was 0.199 wt.%, which is in compliance with the standards (0.25 mol% and 0.24 wt.%, respectively).
The Gas chromatograms of the analyzed biodiesel before and after extraction
are shown in Fig. 4a and b, respectively.
To obtain a precise result from the GC analysis, each sample of biodiesel was
injected three times and an average was taken as the representative value. The
peaks of each component were identified using the relative retention times based
on the retention times of the internal standards. As can be seen from the chromatograms
in Fig. 4, there is no peak for free glycerol after extraction
which means that the DES was successful in removing the free glycerol completely.
GC analysis results revealed that all DESs (DES1 to DES8) were able to remove
all free glycerol content.
After identifying the peaks of mono, di and triglycerides and measuring the areas of the peaks, the weight percentage of each glycerides was calculated. The total glycerol was calculated according to the ASTM D-6584 standard as shown in Eq. 1:
||Total glycerol removed by ChCl:ethylene glycol DES
where, G, MG, DG and TG are free glycerol, monoglycerides, diglycerides and triglycerides, respectively.
The effect of DES to biodiesel molar ratios on the removal of total glycerol was also studied. According to Eq. 1, removal of free glycerol reduces the value of the total glycerol. As indicated by Fig. 5, the ability of DES1, DES2, DES3 and DES4 to decrease the total glycerol content increased with the increase in the DES to biodiesel ratio for all DESs until the ratio of 1:1 is reached. After this ratio, the decrease in total glycerol content was not noticeable. The maximum removal of total glycerol (30 wt. %) occurred at the 2.5:1 DES4 to biodiesel ratio. However, based on the initial amount of solvent and total glycerol removal, DES to biodiesel ratio of 1:1 is chosen as the optimum ratio for the ethylene glycol based DES.
Figure 6, the 2,2,2-trifluracetamide DESs (DES5, DES6, DES7 and DES8) showed their ability to reduce total glycerol content. The figure indicates a considerable reduction in total glycerol at a DES: biodiesel molar ratio of 1:1. After that point no significant change was observed. The maximum removal of total glycerol took place at 3:1 molar ratio using DES6.
The optimum DES to biodiesel ratio for the removal of total glycerol occurs
at 1:1 as well as 2.5:1 and 3:1 for the other two respective DES systems tested.
Looking at the gain in removal between the 1:1 ratio and the other two ratios
reveals that the change is 3.7 and 4.7%, respectively. From a practical point
of view this gain is negligible compared to the difference in amount of solvent
(DES) used for the two mentioned ratios. Hence, to minimize the amount of DES
used in the separation, it was decided to regard the 1:1 ratio as the optimum
separation ratio for both DES systems and neglect the little separation gain
achieved using the other ratios.
||Total glycerol removed by ChCl: 2,2,2-trifluracetamide DES
The current study presented a novel purification method for the removal of glycerol from palm oil based biodiesel. Both DES combination of ChCl:ethylene glycol and ChCl:2,2,2-trifluracetamide were found to be successful to remove all free glycerol from palm oil-based biodiesel. The most effective conditions for reducing total glycerol content for ChCl:ethylene glycol were 1:2.5 salt:HBD and of 2.5:1 DES to biodiesel molar ratios. While for ChCl:2,2,2-trifluracetamide DESs the most effective conditions for reducing total glycerol content were 1:1.75 salt:HBD and 3:1 DES to biodiesel mole ratios. The optimum molar ratio for all DESs (DES1 to DES8) was found to be 1:1 molar ratio of DES to biodiesel.
This study was supported by the University of Malaya IPPP grant number PS133/2009C.