Hakan Turhan
�anakkale Onsekiz Mart University, Turkey
ABSTRACT
Ethylene produced by tissue, callus and plantlets in closed vessels may lead to abnormal plantlet growth and branching in vitro. Silver nitrate (AgNO3) is known as an ethylene inhibitor. Therefore, the objective of this study was to determine the effect of AgNO3 on potato plantlets with measuring several characters. The results showed that MS basal medium supplemented with AgNO3 resulted in an inhibitory effect on ethylene gas produced by potato plantlets. The response of the cultivars used showed genotypic dependence to different AgNO3 concentrations. In general, the best results for all cultivars were obtained from 5 or 10 μM AgNO3. On the other hand, the higher AgNO3 concentrations (25 and 50 μM) can be also used for cultivar Nicola and Desiree that initially had branching and abnormal plantlet growth.
PDF References Citation
How to cite this article
Hakan Turhan, 2004. The Effect of Silver Nitrate (Ethylene inhibitor) on in vitro Shoot
Development in Potato (Solanum tuberosum L.). Biotechnology, 3: 72-74.
DOI: 10.3923/biotech.2004.72.74
URL: https://scialert.net/abstract/?doi=biotech.2004.72.74
DOI: 10.3923/biotech.2004.72.74
URL: https://scialert.net/abstract/?doi=biotech.2004.72.74
REFERENCES
- Mayor, M.L., G. Nestares, R. Zorzoli and L.A. Picardi, 2003. Reduction of hyperhydricity in sunflower tissue culture. Plant Cell Tissue Org. Cult., 72: 99-103.
CrossRefDirect Link - Kotsias, D. and P.A. Roussos, 2001. An investigation on the effect of different plant growth regulating compounds in in vitro shoot tip and node culture of lemon seedlings. Scientia Horticulturae, 89: 115-128.
CrossRefDirect Link - Mohiuddin, A.K.M., M.K.U. Chowdhury, Z.C. Abdullah and S. Napis, 1997. Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell Tissue Organ Cult., 51: 75-78.
Direct Link - Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
CrossRefDirect Link - Brar, M.S., M.J. Moore, J.M. Al-Khayri, T.E. Morelock and E.J. Anderson, 1999. Ethylene inhibitors promote in vitro regeneration of cowpea (Vigna unguiculata L.). In vitro Cell. Dev. Biol. Plant, 35: 222-225.
Direct Link