Variation in Soil Mycobiota Associated with Decomposition of Sesbania aculeata L.
The aim of the present study was to investigate the variation in soil mycobiota involved in the decomposition of Sesbania aculeata L. in soil. Decomposition of Sesbania aculeata (Dhaincha) was studied by nylon net bag technique under experimental conditions. The colonization pattern by soil inhabiting mycobiota was studied by standard methods. Among the three methods used for isolation and enumeration of fungi, dilution plate technique recorded the highest number of fungi followed by damp chamber and direct observation method. Nutrient availability and climatic conditions influenced occurrence and colonization pattern of mycobiota. Maximum fungal population was recorded in July (48.95±0.20x104 of fungi/g oven dry litter) and minimum in June (19.78±0.20x104 of fungi/g oven dry litter). The distribution of Deuteromycetous fungi was much more (74.47%) than Zygomycetes, Oomycetes and Ascomycetes. In the early stage of decomposition Mucor racemosus, Rhizopus stolonifer, Chaetomium globosum and Gliocladium roseum were found where as at the later stages of decomposition preponderance of Aspergillus candidus, Cladosporium cladosporioides, Curvularia lunata and Aspergillus luchuensis was recorded.
June 10, 2010; Accepted: July 15, 2010;
Published: April 28, 2011
Green manures have become a vital concern particularly in the context of sustainable
agriculture. Use of Sesbania as a green manure crop is a common practice
in South Asia. Like most of the green manure crops, Sesbania belongs
to the family Leguminosae and its subfamily is Papilionoideae. Species of the
genus Sesbania are known for exceptionally fast growth rates as well
as a very high affinity for association with several nitrogen-fixing Rhizobia
in the soil that cause formation of numerous and large nodules in the plant
roots. Besides this it is shown to be a rich source of nutrients from organic
to inorganic through various activities of microorganism (Palaniappan,
1994; Hundal et al., 1992; Brar
and Sidhu, 1995; Singh et al., 2007). Effects
of the age on the decomposition period of Sesbania aculeata (Bhardwaj,
1982) and other green manure crop (Atkinson and Cairns,
2001; Wardle et al., 2009) and colonization
pattern of culturable decomposers during decomposition of green manures (Akpor
et al., 2006) have been studied. Crop residue decomposition is essential
for maintaining health of the soil, increasing water holding capacity and improving
soil-water-air conditions. Among the microbes involved in the decomposition,
fungi come under the important group. They grow well under semi-solid fermentation
conditions and colonize it quickly by virtue of their ability to ramify through
solid substrate (Hudson, 1971). The process of decomposition
is governed by the succession of fungi at its various stages (Valenzuela
et al., 2001; Rai et al., 2001; De
Santo et al., 2002; Osono, 2005; Kodsueb
et al., 2008; Gusewell and Gessner, 2009),
nutrient level of soil, crop residue and prevailing environmental conditions
(Cookson et al., 1998; Cruz
et al., 2002; Simoes et al., 2002;
McTiernan et al., 2003; Xingbing
et al., 2009; Wood et al., 2009).
The various groups of soil mycobiota involved in the decomposition of Sesbania
aculeata L. contribute differentially and have stage specific occurrence
in the decomposition process. Therefore the primary aim of present study is
to investigate various soil mycobiota involved in the decomposition of Sesbania
MATERIALS AND METHODS
For the study the decomposition of Sesbania aculeata L. in experimental
conditions by soil mycobiota, the material was collected after harvesting of
the Sesbania crop from the experimental site (Agriculture farm, Institute
of Agricultural Sciences, Banaras Hindu University, Varanasi-221005). Decomposition
was studied by nylon net bag technique (House and Stinner,
1987). The Sesbania (Dhaincha) plants were cut into small pieces
(2-3 cm). Fifty gram of air-dried green manure pieces was filled in each nylon
net bag of 30x25 cm size with mess size of 1-2 mm2. A trench with
an area of 4x4x0.1 m was dug in the field. All nylon net bags were kept in trench
at depth of 10 cm and trench was filled with soil. Sampling programme was run
from July, 2008 to June, 2009 at monthly intervals. Four samples (nylon net
bags) were used for the experiment to determine weight loss, moisture content
and pH and for the isolation of fungi for each month. Different media viz.,
Czapeks dox agar, carboxymethylcellulose agar and Potato dextrose agar
medium were used for screening, isolation and sub-culturing of mycobiota associated
with decomposition. The following three methods were used for the study of inhabiting
mycobiota of Sesbania aculeata as discussed under.
Direct observation method: The fungi on the decomposing Sesbania
aculeata were observed under binocular microscope (Garrett,
Damp chamber incubation method: This method was described by Boeding
(1956). The samples were cut into 1-2 cm pieces and placed on sterilized
blotting paper in Petri dishes. These Petri dishes were incubated at 25±2°C
for 15 days.
Dilution plate technique: Warcup (1960) proposed
this method for isolation and determination of fungal population. Samples were
powdered and 1 g of it was suspended into 10 mL sterilized distilled water.
Further dilution series (1:103, 1:104, 1:105)
were prepared from it. Five replicates with 1 mL of each dilution were incubated
on Czapeks dox agar medium, potato dextrose agar medium and cellulose
methyl agar medium with 100 ppm streptomycin at 25±2°C for 6-7 days
and fungi were recorded. This method was repeated at monthly interval to observe
the monthly occurrence of decomposing mycobiota. Total number of fungi/g of
oven dried sample was calculated.
Statistical analysis: The data was analyzed using CRD design and result was expressed in terms of LSD (least significant difference).
|| Meteorological data standard (month wise) of Varanasi during
The fungal species were identified with the help of literature available (Thom
and Raper, 1945; Raper and Thom, 1949; Ellis,
1976; Barnett and Hunter, 1972; Gilman,
1975). Moisture content was determined by drying the samples at 60°C
for 24 h and subtracting this value from initial weight of the respective value.
The pH of samples was determined with the help of Elico-Electric pH meter and
weight loss by means of litter weight technique (Bocock,
1964). Meteorological data (Table 1) showing maximum and
minimum temperature, relative humidity and rainfall were obtained from meteorological
observatory, Department of Agronomy, Institute of Agricultural Sciences, Banaras
Hindu University, Varanasi, India.
The monthly and progressive weight loss of Sesbania aculeata samples during decomposition is given in Table 2, the loss in weight of substrate was recorded throughout the decomposition period but was maximum (30.60%) in January. Higher weight loss (28.61%) was also recorded in November. Fluctuating moisture content, pH and average number of fungi/g oven dry litter is presented in Table 3. The maximum number (48.95±0.20x104 of fungi/g oven dry litter) of fungi was recorded in July and minimum (19.78±0.20x104 of fungi/g oven dry litter) in June. The pH varied from 5.69±0.01 to 7.21±0.02 with no definite trend. Moisture content showed climatic factors associated variation throughout the decomposition period. The summer months remained almost dry and led to the gradual decrease in moisture content of substrate from January to April. There was gradual decrease in population of fungi from July to December and marginal increase in population in January. Thereafter, there was sharp decrease from February to June.
The number of fungal species isolated from decomposing Sesbania aculeata L. is given in Table 4, a total of 42 fungal species were isolated by dilution plate technique, 23 fungal species by damp chamber method and 16 by direct observation method. Dominant fungal species were Aspergillus flavus, A. niger, A. fumigatus, Penicillium rubrum, Trichroderma harzianum, Fusarium semitectum and dark sterile mycelium.
The common fungi observed during the study were Cladosporium cladosporioides,
Fusarium species, Penicillium citrinum, Aspergillus luchuensis,
Curvularia lunata, Aspergillus terreus, A. sydowi, Nigrospora
sphaerica and Alternaria alternata.
|| Weight loss of Sesbania aculeata L. samples during
|Values are mean (n = 3). **Additive value of subsequent months
||pH, moisture content and average number of fungi per g oven
dry decomposing green manure (Sesbania aculeata L.) under experimental
|Values are mean (n = 3) ±SD
Rare occurring fungi were Epicoccum purpurascens, Torula graminis,
Mortirella subtilissima and Choanephora cucurbitarum. Some decomposing
mycobiota viz., Aspergillus niger, A. flavus, Penicillum rubrum,
Trichoderma harzianum, Cladosporium cladosporioides, Alernaria
alternata, Curvularia lunata and Fusarium semitectum were
found throughout the decomposing period. These fungi were designated as dominant
decomposing mycobiota. Data contained in Table 5 revealed
the class wise distribution of fungal communities involved in the decomposition
of Sesbania aculeata L. Among the recorded fungal communities the Deuteromycetous
fungi constituted 74.47% of total fungal population followed by Zygomycotina,
Mastigomycotina and Ascomycotina, respectively.
|| Fungi recorded during decomposition of green manure of Sesbania
|1Direct observation method; 2Damp chamber
method; 3Dilution plate technique. +: Present, -: Absent
||Class wise occurrence of fungi and per cent occurrence of
various classes colonizing the decomposing Sesbania aculeata L. under
Maximum weight loss was recorded in January, 2009. It may be attributed to
increased microbial activity due to favourable atmospheric temperature (Table
1), optimum soil moisture and soil pH (Table 2) condition
owing to rainfall towards end of January. The significant correlation with weight
loss and rate of decomposition owing to environmental factors were reported
earlier by Cookson et al. (1998) and Beare
et al. (2002). Salamanca et al. (2003)
observed that decrease in weight due to leaching effect of rainfall and synergistic
action of microbes and soil fauna.
Optimum soil moisture content affects the marked increase in soil fauna, its
distribution and colonization on substrate was reported by Beare
et al. (2002). Whereas, pH increases in soil due to incorporation
of higher biomass into the soil to increase the bioactivity thereby, resulting
in weight loss (Zimmermann and Frey, 2002).
The maximum fungal population was recorded in July. It may be attributed to
senescent residues provided enough moribund tissues and the surface area for
the activities of initial colonizers to allow the succession which are unable
to appear on fresh decaying tissue and narrow C:N ratio. Rate of decomposition
of substrate in soil after incorporation remained higher in the first and second
week, which gradually slowed down and finally become steady owing to decline
in fungal population (Berkenkamp et al., 2002).
Sariyildiz and Anderson (2003) reported that the decomposition
of organic amendment were initially rapid and then plateaued. The significance
of increasing temperature and decreasing relative humidity of air, resulting
in decline of fungal population during summer months has also been earlier reported
by Khanna (1964) and Cruz et al.
(2002). While, McTiernan et al. (2003) observed
that wet and warm climatic conditions had more recalcitrant effect on litter
decomposition. In the last stage of decomposition fungal colonization is mainly
governed by nutritional level rather than environmental conditions (Ambus
and Jensen, 1997; Cookson et al., 1998; Osono,
2005; Gusewell and Gessner, 2009).
The distribution of higher percentage of Deuteromyceteous fungi suggested that
the fungi belonging to this class are strong colonizers of the decaying substrate
with better adaptability, high competitive ability and their higher percentage
of distribution whereas, those of Phycomycetes and Ascomycetes were weak colonizers
was reported by several workers (Rai et al., 2001;
De Santo et al., 2002; Vibha
and Sinha, 2007). These reports support the experimental findings of the
present study. The order of fungal succession upon a natural substrate reflects
the sequential release of different organic and inorganic nutrients along with
interaction between each individual and substratum besides the competition between
individual fungi (Hobbie et al., 2003; Kodsueb
et al., 2008).
Authors thank Head, Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi for laboratory facilities. Their sincere thanks are also due to University Grant Commission, Govt. of India, for the financial support.
Akpor, O.B., A.I. Okoh and G.O. Babalola, 2006. culturable microbial population dynamics during decomposition of Theobroma cacao leaf litters in a tropical soil setting. J. Boil. Sci., 6: 768-774.
CrossRef | Direct Link |
Ambus, P. and E.S. Jensen, 1997. Nitrogen mineralization and denitrification by crop residue particle size. Plant Soil, 197: 261-270.
Atkinson, R.B. and J. Cairns, 2001. Plant decomposition and litter accumulation in depressional wetlands: Functional performance of two wetland age classes that were created via excavation. Wetlands, 21: 354-362.
Barnett, H.L. and B.B. Hunter, 1972. Illustrated Genera of Imperfect Fungi. Burgess Publishing Co., Minnesota.
Beare, M.H., P.E. Wilson, P.M. Fraser and R.C. Butler, 2002. Management effects on barley straw decomposition, nitrogen release and crop production. Soil Sci. Soc. Am. J., 66: 848-856.
Direct Link |
Berkenkamp, A., E. Priesack and J.C. Munch, 2002. Modeling the mineralization of plant residue on the soil surface. Agronomie, 22: 711-722.
Bhardwaj, K.K.R., 1982. Effect of the age and decomposition period of dhaincha on the yield of rice. Indian J. Agron., 27: 284-285.
Bocock, K.L., 1964. Changes in the amounts of dry matter, nitrogen, carbon and energy in decomposing woodland leaf litter in relation to the activities of the soil fauna. J. Ecol., 52: 273-284.
Direct Link |
Boeding, K.B., 1956. Trypon blue as a stain of fungi. Stain Technol., 31: 115-116.
Direct Link |
Brar, D.S. and A.S. Sidhu, 1995. Effect of soil water on patterns of nitrogen release during decomposition of added green manure residue. J. Indian Soc. Soil Sci., 43: 14-17.
Cookson, W.R., M.H. Beare and P.E. Wilson, 1998. Effects of prior crop residue management on microbial properties and crop residue decomposition. Applied Soil Ecol., 7: 179-188.
Cruz, A.G., S.S. Gracia, F.J.C. Rojas and A.I.O. Ceballos, 2002. Foliage decomposition of velvet bean during seasonal drought. Interciencia, 27: 625-630.
Direct Link |
De Santo, A.V., F.A. Rutigliano, B. Berg, A. Fioretto, G. Puppi and A. Alfuni, 2002. Fungal mycelium and decomposition of needle litter in three contrasting coniferous forests. Acta Oecol., 23: 247-259.
Ellis, M.B., 1976. More Dematiaceous Hyphomycetes. 1st Edn., Commonwealth Mycological Institute, Kew, Surrey, UK., Pages: 507.
Garrett, S.D., 1981. Soil Fungi and Soil Fertility. 2nd Edn., The Macmillan Company, Oxford, New York.
Gilman, J.C., 1975. A Manual of Soil Fungi. Oxford and IBH Publication Co., New Delhi.
Gusewell, S. and M.O. Gessner, 2009. N: P ratios influence litter decomposition and colonization by fungi and bacteria in microcosms. Funct Ecol., 23: 211-219.
Hobbie, E.A., L.S. Watrud, S. Maggard, T. Shiroyama and P.T. Rygiewicz, 2003. Carbohydrate use and assimilation by litter and soil fungi assessed by carbon isotopes and BIOLOG (R) assays. Soil Biol. Biochem., 35: 303-311.
House, G.J. and R.E. Stinner, 1987. Decomposition of plant residues in no-tillage agroecosystem: Influence of litterbag mesh size and soil arthropods. Pedobiologia, 30: 351-360.
Direct Link |
Hudson, H.J., 1971. Fungal Saprophytism Studies in Biology. Edward Arnold Publisher, London.
Hundal, H.S., N.S. Dhillon and G. Dev, 1992. Contribution of different green manures to P nutrition of rice. Indian J. Soil Sci. Soc., 40: 76-81.
Khanna, P.K., 1964. The Succession of fungi on some decaying grasses. Ph.D. Thesis, Banaras Hindu University, Varanasi, India.
Kodsueb, R., E.H.C. McKenzie, S. Lumyong and K.D. Hyde, 2008. Fungal succession on woody litter of Magnolia liliifera (Magnoliaceae). Fungal Diversity, 30: 55-72.
Direct Link |
McTiernan, K.B., M.M. Couteaux, B. Berg, M.P. Berg and R.C. de Anta et al., 2003. Changes in chemical composition of Pinus sylvestris needle decomposition along a European coniferous forest climate transect. Soil Biol. Biochem., 35: 801-812.
Osono, T., 2005. Colonization and succession of fungi during decomposition of Swida controversa leaf litter. Mycologia, 97: 589-597.
Direct Link |
Palaniappan, S.P., 1994. Green Manuring: Nutrient Potential and Management. In: Fertilizers, Organic Manure, Recyclable Waste and Biofertilizers, Tandon, H.L.S. (Eds.). Fertilizer Development and Consultation Organization, New Delhi.
Rai, J.P., A. Sinha and S.R. Govil, 2001. Litter decomposition mycoflora of rice straw. Crop Res., 21: 335-340.
Raper, K.B. and C. Thom, 1949. A Manual of Penicillia. Williams Wilkins Company, Baltimore, pp: 875.
Salamanca, E.F., M. Kakeno and S. Katagiri, 2003. Rainfall manipulation effect on litter decomposition and the microbial biomass of forest floor. Applied Soil Ecol., 22: 271-281.
Direct Link |
Sariyildiz, T. and J.M. Anderson, 2003. Decomposition of sun and shade leaves from three deciduous tree species, as affected by their chemical composition. Biol. Fert. Soil, 37: 137-146.
CrossRef | Direct Link |
Simoes, M.P., M. Madeira and L. Gazariani, 2002. Decomposition dynamics and nutrient release of Cistus salvifolius L. and Cistus ladonifer L. leaf litter. Rev. Ciencias Agrarias, 25: 508-520.
Singh, S., N. Ghoshal and K.P. Singh, 2007. Synchronizing nitrogen availability through application of organic inputs of varying resource quality in a tropical dryland agroecosystem. Applied Soil Ecol., 36: 164-175.
Thom, C. and K.B. Raper, 1945. A Manual of Aspergilli. Williums and Wilkins Co., USA.
Valenzuela, E., S. Leiva and R. Godoy, 2001. Seasonal variation and enzymatic potential of microfun gi associated with the decomposition of Northofagus pumilio leaf litter. Revista Chilena Historia Natural, 74: 737-749.
Vibha and A. Sinha, 2007. Variation of soil mycoflora of rice stubble from rice wheat cropping system. Mycobiology, 35: 191-195.
Warcup, J.H., 1960. Method for Isolation and Estimation of Activities of Fungi in Soil. In: Ecology of Soil Fungi, Parkinson, D. and J.S. Waids (Eds.). The University Press, Liverpool, UK.
Wardle, D.A., R.D. Bardgett, L.R. Walker and K.I. Bonner, 2009. Among and within species variation in plant litter decomposition in contrasting long-term chronosequences. Funct Ecol., 23: 442-453.
Wood, T.E., D. Lawrence, D.A. Clark and R.L. Chazdon, 2009. Rain forest nutrient cycling and productivity in response to large-scale litter manipulation. Ecology, 90: 109-121.
Xingbing, H., P. Zhang, Y. Lin, A. Li, X. Tian and Q.H. Zhang, 2009. Responses of litter decomposition to temperature along a chronosequence of tropical montane rainforest in a microcosm experiment. Ecol. Res., 24: 781-789.
Zimmermann, S. and B. Frey, 2002. Soil respiration and microbial properties in acid forest soil: Effect of wood ash. Soil Biol. Biochem., 34: 1727-1737.
Direct Link |