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    Publisher: Asian Network for Scientific Information
   
  Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.

Submit your best paper to PJBS via online submission system.
  Editor-in-Chief:  Hakan UlUKAN
 
 
Ahmad, I., M. Irfan, M. Nadeem, M.A. Zia, B.M. Ahmad and H.M.N. Iqbal, 2010. Relationship of process parameters for the production of alkaline protease by Bacillus sp. Int. J. Agr. Vet. Med. Sci., 4: 105-113.
Ahsan, T., J. Chen, X. Zhao, M. Irfan and Y. Wu, 2017. Extraction and identification of bioactive compounds (Eicosane and Dibutyl phthalate) produced by Streptomyces strain KX852460 for the Biological control of Rhizoctonia solani AG-3 strain KX852461 to control Target spot disease in tobacco leaf. AMB Exp., Vol. 7 10.1186/s13568-017-0351-z.
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Ahsan, T., J. Chen, Y. Wu and M. Irfan, 2017. Application of response surface methodology for optimization of medium components for the production of secondary metabolites by Streptomyces diastatochromogenes KX852460. AMB Exp. 10.1186/s13568-017-0388-z.
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Ahsan, T., J. Chen, Y. Wu, M. Irfan and J. Shafi, 2017. Screening, identification, optimization of fermentation conditions and extraction of secondary metabolites for the biocontrol of Rhizoctonia solani AG-3. Biotechnol. Biotechnol. Equipment, 31: 91-98.
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Arooj, A., M. Irfan, F. Tabsum, H.A. Shakir and J.I. Qazi, 2017. Effect of dilute sulphuric acid pretreatment on cellulase production by Bacillus subtilis K-18 through response surface methodology. Proc. Pak. Acad. Sci. B: Life Environ. Sci., 54: 11-20.
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Asghar, U., M. Irfan, M. Iram, Z. Huma, R. Nelofer, M. Nadeem and Q. Syed, 2015. Effect of alkaline pretreatment on delignification of wheat straw. Nat. Prod. Res., 29: 125-131.
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Asghar, U., M. Irfan, M. Nadeem and Q. Syed, 2016. Effect of NaOH on pretreatment of gossypium herbaceum. Energy Sources Part A Recovery Util. Environ. Effects, 38: 1898-1903.
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Asghar, U., M. Irfan, M. Nadeem, R. Nelofer and Q. Syed, 2015. Challenges in bioethanol production from lignocellulosic waste. Energy Educ. Sci. Technol. Part C Future Energy Resour., 7: 7-16.
Asghar, U., M. Irfan, M. Nadeem, R. Nelofer and Q. Syed, 2015. Effect of KOH pretreatment on lignocellulosic waste to be used as substrate for ethanol production. Iran. J. Sci. Technol., .
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Ashgar, U., M. Nadeem, M. Irfan, Q. Syed, R. Nelofer and M. Iram, 2016. Effect of NaOH on delignification of Saccharum spontaneum. Environ. Prog. Sustainable Energy, 35: 284-288.
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Bai, H., H. Wang, J. Sun, M. Irfan and M. Han et al., 2013. Production, purification and characterization of novel beta glucosidase from newly isolated Penicillium simplicissimum H-11 in submerged fermentation. EXCLI J., 12 528.
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Bai, H., H. Wang, J. Sun, M. Irfan and M. Han et al., 2013. Purification and characterization of beta 1, 4-Glucanases from Penicillium simplicissimum H-11. BioResources, 8: 3657-3671.
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Chaudhary, N., J.I. Qazi and M. Irfan, 2015. Isolation and identification of cellulolytic and ethanologenic bacteria from soil. Iran. J. Sci. Technol., .
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Chen, L., Z. Li, W. Qi, M. Irfan and J. Lin et al., 2017. Characterization of promoter region of glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum. Turk. J. Biol., Vol. 41. .
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Deeba, F., H.A. Shakir, M. Irfan and J.I. Qazi, 2016. Chitinase production in organisms: A review. Punjab Univ. J. Zool., 31: 101-106.
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Gulsher, M., M. Nadeem, Q.A. Syed, M. Irfan and S. Baig, 2010. Alkaline protease production from industrial waste by Bacillus subtilis ML-4. Pak. J. Sci. Ind. Res., 53: 146-150.
Gulsher, M., S. Abbas, M. Irfan, M. Nadeem, Q.A. Syed and S. Baig, 2010. Biosynthesis of cellulase from bagasse with fungal strains. Pak. J. Biochem. Mol. Biol., 43: 146-148.
Hayat, M.U., M. Irfan, A.A.N. Saqib, M. Nadeem, Q.A. Syed and S. Baig, 2010. Degradation of cellulose by co-cultures and the mixture of culture filtrates. Sci. Int. (Lahore)., 22: 303-306.
Hu, M., X. Zhou, Y. Shi, J. Lin, M. Irfan and Y. Tao, 2014. Essential role of the N-and C-terminals of laccase from Pleurotus florida on the laccase activity and stability. Applied Biochem. Biotechnol., 174: 2007-2017.
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Iqbal, H.M.N., I. Ahmed, M.A. Zia and M. Irfan, 2011. Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility. Adv. Biosci. Biotechnol., 2: 149-156.
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Iqbal, M.N., A.A. Anjum, M.A. Ali, F. Hussain and S. Ali et al., 2015. Assessment of microbial load of un-pasteurized fruit juices and in vitro antibacterial potential of honey against bacterial isolates. Open Microbiol. J., 9: 26-32.
Irfan, M. and Q. Syed, 2011. Effect of 2-mercaptoethanol on enzymes secretion by Aspergillus niger in submerged fermentation. Pak. J. Biochem. Mol. Biol., 44: 160-163.
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Irfan, M. and Q. Syed, 2012. Partial purification and characterization of xylanase from Trichoderma viride produced under SSF. Int. J. Appl. Res. Nat. Prod., 5: 7-11.
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Irfan, M., 2011. Trichoderma sp. A potent producer of xylanase enzyme: Xylanase production in solid state fermentation. Lap Lambert Academic Publishing GmbH and Co. KG, Germany., ISBN: 978-3847316176, Pages: 72..
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Irfan, M., A. Safdar, Q. Syed and M. Nadeem, 2012. Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Turk. J. Biochem., 37: 287-293.
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Irfan, M., A., Rauf, Q.A. Syed, M. Nadeem and S. Baig, 2011. Exploitation of different agro-residues for acid protease production by Rhizopus sp. in Koji fermentation. Int. J. Agr. Vet. Med. Sci., 5: 43-52.
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Irfan, M., J. Javed and Q. Syed, 2011. UV mutagenesis of Aspergillus niger for enzyme production in submerged fermentation. Pak. J. Biochem. Mol. Biol., 44: 137-140.
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Irfan, M., J.X. Sun, Y. Liu, X. Li and S. Yang, 2014. Genetic analysis of chlorophyll content in maize by mixed major and polygene models. Genetika, 46: 1037-1046.
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Irfan, M., M. Gulsher, M. Nadeem and Q.A. Syed, 2009. Evaluation of cultural conditions for thermostable α-amylase production from Bacillus sp. production. Pak. J. Biochem. Mol. Biol., 42: 43-48.
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Irfan, M., M. Gulsher, M. Nadeem, Q.A. Syed and S. Baig, 2009. Submerged production of alkaline protease by newly isolated Bacillus sp. Pak. J. Food Sci., 19: 12-16.
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Irfan, M., M. Gulsher, S. Abbas, Q. Syed, M. Nadeem and S. Baig, 2011. Effect of various pretreatment conditions on enzymatic saccharification. Songklanakarin J. Sci. Technol., 33: 397-404.
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Irfan, M., M. Nadeem and Q. Syed, 2012. Influence of nutritional conditions for endoglucanase production by Trichoderma viride in SSF. Global J. Biotechnol. Biochem., 7: 7-12.
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Irfan, M., M. Nadeem and Q. Syed, 2012. Media optimization for amylase production in solid state fermentation of wheat bran by fungal strains. J. Cell. Mol. Biol., 10: 55-64.
Irfan, M., M. Nadeem and Q. Syed, 2013. Production of cellulases and hemicellulases from cellulytic fungal cultures in submerged fermentation using agricultural wastes. Electron. J. Biol., 9: 62-66.
Irfan, M., M. Nadeem and Q. Syed, 2013. Purification and characterization of xylanase from Bacillus subtilis. Protein Peptide Lett., 20: 1225-1231.
Irfan, M., M. Nadeem and Q. Syed, 2014. Ethanol production from agricultural wastes using Saccharomyces cervisae. Braz. J. Microbiol., 42: 457-465.
Irfan, M., M. Nadeem and Q. Syed, 2014. One-factor-at-a-time (OFAT) optimization of xylanase production from Trichoderma viride-IR05 in solid-state fermentation. J. Radiat. Res. Applied Sci., 7: 317-326.
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Irfan, M., M. Nadeem, Q. Syed and J.I. Qazi, 2016. Statistical optimization of dilute acid pretreatment of pinus needles to be used as substrate for biofuel production. Energy Sources Part A Recovery Util. Environ. Effects, 38: 1983-1992.
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Irfan, M., M. Nadeem, Q. Syed and S. Baig, 2011. Production of thermostable α-amylase from Bacillus sp. in solid state fermentation. J. Appl. Sci. Res., 7: 607-617.
Irfan, M., M. Nadeem, Q. Syed and S. Baig, 2012. Effect of medium composition on xylanase production by Bacillus subtilis using various agricultural wastes. American-Eurasian J. Agric. Environ. Sci., 12: 561-565.
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Irfan, M., M. Nadeem, Q. Syed, H.A. Shakir and J.I. Qazi, 2016. Study on Some Properties of Calcium-dependent a-Amylase from Bacillus subtilis through submerged fermentation of wheat bran. Chem. Biochem. Eng. Q., 30: 429-437.
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Irfan, M., M. Nadeem, Q. Syed, W. Nawaz, S. Baig and A.M. Qureshi, 2010. Relationship of process parameters for the production of alkaline protease by Bacillus sp. Int. J. Agr. Vet. Med. Sci., 4: 114-120.
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Irfan, M., M. Nadeem, Q.A. Syed and S. Baig, 2010. Submerged cultivation of Aspergillus niger on pretreated sugarcane bagasse. World J. Agric. Sci., 6: 466-472.
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Irfan, M., M. Nadeem, S. Baig, Q.A. Syed and A.M. Qureshi, 2008. Purification and characterization of protease from Bacillus spp. Pak. J. Biochem. Mol. Biol., 41: 129-132.
Irfan, M., M.I. Nazir, M. Nadeem, M. Gulsher, Q.A. Syed and S. Baig, 2011. Optimization of process parameters for the production of single cell biomass of Candida utilis in solid state fermentation. Am-Eurasian J. Agric. Environ. Sci., 10: 264-270.
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Irfan, M., Q. Mushtaq, F. Tabassum, H.A. Shakir and J.I. Qazi, 2017. Carboxymethyl Cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology. AMB Exp., Vol. 7. 10.1186/s13568-017-0331-3.
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Irfan, M., Q. Syed, M. Yousaf, M. Nadeem, S. Baig and S.A. Jafri, 2010. Studies on the pretreatment of wheat straw for improve production of carboxymethyl cellulase by Trichoderma viride- FBL1 in solid state fermentation. Acad. Arena., 2: 18-30.
Irfan, M., Q. Syed, S. Abbas, M. GulSher, S. Baig and M. Nadeem, 2011. FTIR and SEM analysis of thermo-chemical fractionated of Sugarcane Bagasse. Turkish J. Biochem., 36: 322-328.
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Irfan, M., Q.A. Syed, M. Gulsher, S. Abbas, M. Nadeem and S. Baig, 2010. Pretreatment of corncobs for the production of hydrolytic enzymes from Aspergillus niger-IR01. Int. J Agric. Vet. Med. Sci., 4: 81-87.
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Irfan, M., S. Abbas, S. Baig, M. Gulsher, M. Nadeem and Q.A. Syed, 2010. Pretreatment: A potential technique to enhance the Enzymatic hydrolysis. World J. Agric. Sci., 6: 440-445.
Irfan, M., S. Yang, L. Yuxin and J. Sun, 2017. Genetic diversity analysis of Morchella sp. by RAPD. Mol. Biol. Res. Commun., 6: 27-31.
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Irfan, M., U. Asghar, M. Nadeem, H.A. Shakir, J.I. Qazi, R. Nelofer and Q. Syed, 2016. Process optimization for endoglucanase production by Trichoderma harzianum in submerged fermentation. Hacettepe J. Biol. Chem., 44: 87-93.
Irfan, M., U. Asghar, M. Nadeem, R. Nelofer and Q. Syed, 2016. Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation. J. Radiat. Res. Appl. Sci., 9: 139-147.
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Irfan, M., U. Asghar, M. Nadeem, R. Nelofer, Q. Syed, H.A. Shakir and J.I. Qazi, 2016. Statistical optimization of saccharification of alkali pretreated wheat straw for bioethanol production. Waste Biomass Valorization, 7: 1389-1396.
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Irfan, M., U. Irfan, Z. Razzaq, Q. Syed and M. Nadeem, 2011. Utilization of agricultural wastes as a substrate for Carboxymethyl cellulase production from Aspergillus niger in submerged fermentation. IJAVMS, 5: 464-471.
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Irfan, M., Z.T. Ting, W. Yang, Z. Chunyu and M. Qing et al., 2013. Modification of CTAB protocol for maize genomic DNA extraction. Res. J. Biotechnol., 8: 41-45.
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Jia B., X. Zhao, Y. Qin, M. Irfan and T.H. Kim, 2016. Identification of quantitative trait loci for leaf traits in rice. Genetika, 48: 643-652.
Karim, A., W. Ali, N. Ahmad, M. Irfan and H.A. Shakir, 2016. Histological and Biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin). Punjab Univ. J. Zool., 31: 229-236.
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Khokhar, Z., Q. Syed, M. Nadeem, M. Irfan and J. Wu et al., 2014. Enhanced production of cellulase by Trichoderma reesei using wheat straw as a carbon source. World Applied Sci. J., 30: 1095-1104.
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Khokhar, Z., Q. Syed, M. Nadeem, S. Baig and M. Irfan et al., 2010. Delignification of wheat straw with acid and hydro-steam under pressure. World Appl. Sci. J., 11: 1524-1530.
Khokhar, Z., Q.A. Syed, S. Baig, M. Nadeem, M. Irfan and M.A. Athar, 2010. Study on filtrate obtained from batch-wise pretreatment of wheat straw at different concentrations of h2so4 and autoclave time. World Applied Sci. J., 10: 1047-1055.
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Li, C., M. Irfan, C. Zhang and F. Lin, 2014. Genetic diversity analysis of maize varities based on SSR markers. Res. J. Biotechnol., 9: 48-51.
Li, J., Y. Yi, X. Cheng, D. Zhang and M. Irfan, 2015. Study on the effect of magnetic field treatment of newly isolated Paenibacillus sp. Botanical Stud., Vol. 56. 10.1186/s40529-015-0083-9.
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Mahmood, S., M.G. Shahid, M. Nadeem, M. Irfan and Q. Syed, 2016. Production and optimization of α-amylase from Aspergillus niger using potato peel as substrate. Pak. J. Biotechnol., 13: 101-109.
Majeed, H.S., M. Irfan, H.A. Shakir and J.I. Qazi, 2016. Filter paper activity producing potential of Aeromonas species isolated from the gut of Labeo rohita. Pak. J. Zool., 48: 1317-1323.
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Munawar, R.A., M. Irfan, M. Nadeem, Q.A. Syed and Z.H. Siddique, 2010. Biosynthesis of single cell biomass of Candida utilis by submerged fermentation. Pak. J. Sci., 62: 1-5.
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Mushtaq, Q., M. Irfan, F. Tabssum and J.I. Qazi, 2016. Potato peels: A potential food waste for amylase production. J. Food Process Eng., 10.1111/jfpe.12512.
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Mushtaq, Z., M. Irfan, M. Nadeem, M. Naz and Q. Syed, 2015. Kinetics study of extracellular detergent stable alkaline protease from rhizopus oryzae. Braz. Arch. Biol. Technol., 58: 175-184.
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Nadeem, A., Q. Syed, S. Baig, M. Irfan and M. Nadeem, 2010. Enhanced production of citric acid by Aspergillus niger M-101 using lower alcohols. Turkish J. Biochem., 35: 7-13.
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Nadeem, A., S.A. Jafri, S. Baig, M. Irfan and Q.A. Syed, 2009. Optimization of substrate concentration for enhanced citric acid production using Aspergillus niger M-101. Pak. J. Sci. Ind. Res., 52: 275-277.
Nadeem, M., M.U. Aftab, M. Irfan, M. Mushtaq, A. Qadir and Q. Syed, 2015. Production of ethanol from alkali-pretreated sugarcane bagasse under the influence of different process parameters. Front. Life Sci., 8: 358-362.
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Rauf, A., M. Irfan, M. Nadeem, I. Ahmed and H.M.N. Iqbal, 2010. Optimization of growth conditions for acidic protease production from Rhizopus oligosporus through solid state fermentation of sunflower meal. World Acad. Sci. Eng. Technol., 72: 608-611.
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Shahid, Z.H., M. Irfan, M. Nadeem, Q. Syed and J.I. Qazi, 2016. Production, purification, and characterization of carboxymethyl cellulase from novel strain Bacillus megaterium. Environ. Prog. Sustainable Energy, 35: 1741-1749.
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Shakir, H.A., J.I. Qazi, M. Irfan and S. Ali, 2016. Alteration in biochemical response of Indian major carp Labeo rohita exposed to urban effluents discharged in the river Ravi Pakistan. Biologia, 62: 219-227.
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Sher, M.G., M. Nadeem, Q. Syed, M. Irfan and S. Baig, 2012. Protease production from UV mutated Bacillus subtilis. Bangladesh J. Sci. Ind. Res., 47: 69-76.
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Siddique, A., A. Gul, M. Irfan, M. Nadeem and M. Syed, 2017. Comparison of different pretreatment methods for efficient conversion of bagasse into ethanol. Biofuels, 8: 135-141.
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Siddique, S., H.A. Shakir, J.I. Qazi, A.B. Tabinda and M. Irfan, 2016. Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1. Punjab Univ. J. Zool., 31: 31-37.
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Siddique, S., Q. Syed, A. Adnan, M. Nadeem, M. Irfan and F.A. Qureshi, 2013. Production of avermectin B1b from Streptomyces avermitilis 41445 by batch submerged fermentation. Jundishapur J. Microbiol., Vol. 6. 10.5812/jjm.7198.
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Su, D., J. Fu and M. Irfan, 2012. First Record of Didymella anemones sp. nov. Teleomorph of Ascochyta anemones, the Causal Agent of Leaf Spots on Pulsatilla korreana in China. J. Phytopathol., 160: 728-731.
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Sun, S., N. Yang, L. Chen, M. Irfan, X. Zhao and T. Li, 2015. Characterization of LpGPAT gene in Lilium pensylvanicum and response to cold stress. Biomed. Res. Int., Vol. 2015. 10.1155/2015/792819.
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Syed, Q.A., M. Irfan, M. Nadeem and S. Baig, 2009. Production of neutral proteases from rice bran using Aspergillus oryzae in solid state fermentation. Pak. J. Biochem. Mol. Biol., 42: 147-152.
Tabssum, F., M. Irfan, H.A. Shakir and J.I. Qazi, 2017. Statistical optimization for deconstruction of poplar substrate by dilute sulphuric acid for bioethanol production. Green Chem. Lett. Rev., 10: 69-79.
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Wang, D., H. Wang, M. Irfan, M. Fan and F. Lin, 2014. Structure and evolution analysis of pollen receptor-like kinase in Zea mays and Arabidopsis thaliana. Comput. Biol. Chem., 51: 63-70.
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Wang, H., X. Zhao, C. Liu, L. Liu and S. Yu et al., 2015. Effects of the biocontrol agent Aspergillus flavipes on the soil microflora and soil enzymes in the rooting zone of pepper plants infected with Phytophthora capsici. J. Phytopathol., 163: 513-521.
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Wang, N., W. Hong, H.Y. Liu, M. Irfan, S.W. Niu and Y. Tao, 2016. Diversity of methane-producing microbial community structure with 16S-V3 rDNA PCR-DGGE. Genetika, 48: 1111-1119.
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Wang, Y., S. Yang, M. Irfan, C. Zhang, Q. Sun, S. Wu and F. Lin, 2013. Genetic analysis of carbon metabolism-related traits in maize using mixed major and polygene models. Aust. J. Crop Sci., 7: 1205-1211.
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Wang, Y., W. Liu, Y. Jiang, L. Huang and M. Irfan et al., 2015. Morphological and molecular characterization of Nosema pernyi, a microsporidian parasite in Antheraea pernyi. Parasitol. Res., 114: 3327-3336.
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Yang, S., M. Irfan, X. Li, Y. Liu and X. Sun, 2013. Detection of Bt gene and seed purity in maize. Hacettepe J. Biol. Chem., 41: 341-346.
Yousaf, M., M. Irfan, Z.U. Khokhar, Q.A. Syed, S. Baig and A. Iqbal, 2010. Enhanced production of Protease by mutagenized strain of Aspergillus oryzae in solid substrate fermentation of rice bran. Sci. Int. (Lahore)., 22: 119-123.
Zhou, T., M. Fan, M. Irfan, H. Wang and D. Wang et al., 2014. Phylogenetic analysis of STK gene family and Usp domain in maize. Mol. Biol. Rep., 41: 8273-8284.
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