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by
Xintian Wen |
Total Records (
3 ) for
Xintian Wen |
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Yong Huang
,
Xianqi Huang
,
Mingxing Tian
,
Nianli Zou
,
Sanjie Cao
and
Xintian Wen
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According to the published sequence of 14 avian Pasteurella multocida genes required for survival and expression in vivo, the swine P. multocida infected models in septicemic mice were established and 14 pairs of primers were designed and synthesized to amplify the corresponding gene of swine P. multocida CVCC 432 strain. Successfully, two genes, pm0221 and pm1069 were identified as in vivo-expression gene using RT-PCR. Their nucleotide sequences were compared with those of avian P. multocida pm70 strain and the homology were 98.8 and 94.8%, respectively. |
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Nianli Zou
,
Fangfang Zhao
,
Ping Liu
,
Sanjie Cao
,
Xintian Wen
and
Yong Huang
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The serotypes and genotypes of Infectious Bronchitis Virus (IBV) was mainly determined by the Spike (S)1 glycoprotein. A prevalent IBV strain ZY3 was isolated and the highly antigenic region of its S1 gene was amplified and expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level was similar antigenically to the native S1 protein as determined by Western blot assay using rabbits polyclonal antibodies against IBV ZY3 strain. The fusion protein was also purified. This research lays the foundation for using this recombinant protein for development of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for serum antibody detection or for production of monoclonal antibodies against prevalent IBV. |
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Min Li
,
Yuanping Wang
,
Nianli Zou
,
Sanjie Cao
,
Xintian Wen
and
Yong Huang
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Interleukin-6 (IL-6) is a multifunctional cytokine which has a wide range of activity in animals and has an impact on almost all immune system cells. To produce specific antibody against chicken IL-6 and detect its activity, its complete Open Reading Frame (ORF) sequence was amplified and inserted into the eukaryotic expression vector pcDNA3.1 (+) to get recombinant eukaryotic plasmid pcDNA-ChIL-6 and BALB/c mouse were immunized with this plasmid to produce polyclonal antibody against Chicken IL-6. Then the mature protein coding genes of chicken IL-6 was cloned and inserted into the prokaryotic expression vector pET-32a (+) to get recombinant prokaryotic plasmid pET-ChIL-6, the expression of this fusion protein was successfully induced by Isopropy 1-β-D-thiogalactoside (IPTG) and the recombinant protein was further purified by NTA His•Bind column. The purified recombinant protein could react positively with polyclonal antibody against pcDNA-ChIL-6 in Western-blot and indirect ELISA. |
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