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Articles by T. Tanha
Total Records ( 4 ) for T. Tanha
  M. Fathi , K. Nazer adl , Y. Ebrahim Nezhad , H. Aghdam Shahryar , M. Daneshyar and T. Tanha
  The present study examined the possible role of reactive oxygen species in the pathogenesis of heart failure in broilers. The experiment was conducted with 160, 1 day old male broilers (Ross 308) to investigate to clarify the mechanism of cell injury causing the pathogenesis of PHS syndrome. The chickens were divided in 2 groups of 4 replicates and 20 chicks for any replicate. One group of these chickens was raised in Normal Temperature (NT) treatment and the other group was raised in Cold Temperature (CT) treatment for induce pulmonary hypertension syndrome. Mortality was inspected to determine cause of death and diagnose of heart failure. Hematological, biochemical and pathological tests were used to determine the incidence of PHS including total Red Blood Cell (RBC), Hemoglobin (HGB), Hematocrit (HCT), release of Alanine Transaminase (ALT), Aspartate Transaminase (AST), Lactate Dehydrogenase (LDH) and a best indicator of lipid oxidation subsequent to generated oxidative stress was Malondialdehyde (MDA). Sampling of blood and liver tissue were determined at day 21 and 42. At end of the experiment (week 6), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed; the right ventricle was dissected away from the left ventricle and septum then ratio of Right Ventricle weight to Total Ventricle weight (RV/TV) calculated too. The results of the experiment indicated that there was a significant difference in RBC, HBG at 21 and HGB, RBC, HCT at 42 between groups as CT group had a greater (p<0.05) HGB, RBC at 21 and HBG, RBC, HCT at 42. However, there was no significant difference in ALT, AST and LDH plasma levels between groups at day 21, CT group had greater (p<0.05) levels in AST, ALT and LDH at day 42. The levels malondialdehyde equivalents an indicator of lipid oxidation sub-sequent to generated oxidative stress at plasma and liver tissue was significantly higher (p<0.05) in CT group at day 21 and 42. RV/TV ratio and mortality due to ascites, also were significantly affected by treatments as CT group had greater (p<0.05) RV/TV and mortality due to ascites mortality percentage compared to NT group. In conclusion, the results indicated that the deteriration of heart function in modern fast growing broilers in the experimental model is associated with oxidative stress leading to lipid peroxidation and reactive oxygen species may be involved in the pathogenesis of the pulmonary hypertension syndrome in broilers chickens.
  T. Tanha , H. Amanlou , M. Chamani , Y. Ebrahimnezhad , R. Salamatdost , N. Maheri and M. Fathi
  The objective of this study was to investigate whether consuming of protected glutamine before and after parturition would affect biomarkers of oxidative stress, dry matter intake and production performance. Thirty six pregnant Holstein dairy cows were assigned into four treatment groups based on their BCS, parity and expected calving date in a completely randomized design. Treatment groups consisted of glutamine supplementation Before and After Calving (BFAF), glutamine supplementation before calving and without glutamine after it (BFAN) without glutamine before and glutamine supplementation after calving and (BNAF) and without glutamine pre and postpartum (BNAN). There were not any significant differences and interaction among treatments in DMI but DMI on days 21 was affected by post partum glutamine feeding. Milk yield was not affected by treatments. The Total Antioxidant Status (TAS) influenced by postpartum glutamine feeding and was most for BFAF and BNAF. The Plasma Glutathione Activity (PGA) was affected by pre and postpartum treatments and the effect of post calving feeding was more clear and there was only an interaction effect on 7 days.
  M. Fathi , K. Nazeradl , Y. Ebrahim Nezhad , H. Aghdam Shahryar , M. Daneshyar and T. Tanha
  Major objectives of this study were to assess antioxidant protection in plasma and whole liver in broilers with pulmonary hypertension syndrome. The experiment was conducted with 160, 1 day old male broilers (Ross 308). The chickens were divided in 2 groups of 4 replicates and 20 chicks for any replicate. One group of these chickens was raised in normal temperature (control without PHS) treatment and the other group was raised in cold temperature (with PHS) treatment for induce pulmonary hypertension syndrome. Mortality was inspected to determine cause of death and diagnose of PHS. For evaluation of antioxidant status, the parameters that were determined in plasma and liver include: Glutathione Peroxidase (GPX), Superoxide Dismutase (SOD), Total Antioxidant Status (TAS) and Malondialdehyde (MDA). Sampling of blood and liver tissue were determined at day 21 and 42. At end of the experiment (week 6), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed, the right ventricle was dissected away from the left ventricle and septum then ratio of Right Ventricle weight to Total Ventricle weight (RV/TV) calculated too. The results of the experiment indicated that in plasma (GPX) and (SOD) activity were elevated in broilers with PHS compared to controls. It was also, PHS group had low TAS compared to control. But in liver, just GPX (not for SOD) was elevated in PHS group than control. The levels malondialdehyde equivalents an indicator of lipid oxidation subsequent to generated oxidative stress at plasma and liver tissue was significantly higher in PHS group compared control. RV/TV ratio and mortality due to ascites, also were significantly affected by treatments as PHS group had greater RV/TV and mortality due to ascites mortality percentage compared to control.
  T. Tanha , H. Amanlou , M. Chamani , Y. Ebrahimnezhad , R. Salamatdost , N. Maheri , M. Fathi and M. Abozar
  The objective of this study was to investigate whether consuming of Protected Glutamine (PG) before parturition in close up period would affect biomarkers of oxidative stress, immune system and Haptoglobin (HP) and Serum Amyloid A (SAA). About 36 pregnant Holstein dairy cows were assigned into two treatment groups based on their BCS and expected calving date in at student examination. Treatment groups consisted of glutamine supplementation 100 g day-1 per cow before calving (F), glutamine did not supplementation before calving (N). There were not any significantdifferences among treatments in DMI and BCS on 21, 14 and 7 days before parturation. There were not significant differences in the Total Antioxidant Status (TAS), Haptoglobin (HP), Serum Amyloid A (SAA), No Esterified Fatty Acids (NEFA) and blood and immune cells. The plasma Glutathione Peroxidase activity (GPX) was significant difference between two group and it seems that supplementation diets with glutamine on the close up period can enhanceplasma Glutathione Peroxidase activity (GPX).
 
 
 
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