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| Articles
by
R. Osfoori |
Total Records (
2 ) for
R. Osfoori |
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M. Ahmadi
,
Y. Mohammadi
,
H. Darmani Kuhi
,
R. Osfoori
and
S. Qanbari
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The polymorphism in the bovine κ-casein (κ-CN)
and β-lactoglobulin (β-LG) genes were analyzed for DNA sequence
variants using the polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP) technique in Iranian Holstein population to find
association between the genotypes and milk traits as well as somatic cell
score. To this purpose, 2537 test day records of one hundred thirty nine
dairy cows were entered in a repeated generalized mixed model. The frequencies
of alleles A and B were estimated as 0.81, 0.19 and 0.57, 043 for κ-CN
and β-LG loci, respectively. The results of association analysis
by the mixed model revealed a significant positive relationship of κ-CN
genotype AA on milk yield (p≤0.04). These analyses did not reveal a
significant association of κ-CN genotypes with fat percent and SCS
traits. In the case of β-LG locus statistical analysis showed a strong
relationship between BB genotype and protein percentage (p≤0.007) comparing
to the other genotypes. These results did not reveal a significant association
of β-LG genotypes with milk yield, milk fat percentage and SCS in
studied animals. A tendency to being significant was however observed
for β-LG genotype AA (p<0.12) with the SCS trait. Because of the
lack of consistency among results of similar studies, we suggest further
investigations to determine the precise nature of these associations with
the milk proteins to be performed based on haplotypes. |
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S. Qanbari
,
M.P. Eskandari Nasab
,
R. Osfoori
and
A. Hagh Nazari
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We report the genetic analysis of 100 individuals of an elite breeding flock of Afshari sheep with a selected set of eighteen microsatellite markers. A full characterization of this set of eighteen loci was carried out generating allele frequency distributions that were used to estimate the genetic information content of these loci, including genetic variability, inbreeding, individual and parent verification parameters. Disregarding MCMA26 monomorphic pattern, microsatellite loci showed moderate level of polymorphism, as such totally 102 alleles were detected with a mean number of 6 alleles per locus. The average expected heterozygosity was 0.72 (SD = 0.07) and the average Polymorphism Information Content (PIC) was 0.67 (SD = 0.08). Total value of inbreeding based on marker data was estimated as -0.02 so it indicates that inbreeding occurred less than would be expected at random. The overall probability of identity considering all twelve independent loci combined was 3.148E-13 meaning lower than 1 in 31 trillions. The results of this study indicate, despite the selective breeding and closed flock system over a number of generations, a relatively high level of heterozygosity still exists in the representative sheep flock. The high degree of multiallelism and the clear and simple codominant Mendelian inheritance of the set of microsatellites used provide a powerful system for the unique identification of Afshari sheep individuals for fingerprinting purposes and parentage testing. |
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