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This field survey was conducted to monitor microbial flora in conjunctiva and compare antibiogram test. Conjunctival swabs from both eyes of 365 cattle that were in different breeds, ages and sexes were sampled. Antibiogram tests were performed using the disc diffusion method. Microorganisms were not isolated from 402 of total 730 conjunctival samples (55%), whereas they were isolated from 328 conjunctival samples (45%). Of these, 352 samples were contaminated with bacteria (99.4%) and 2 samples were contaminated with fungi (0.6%). The sensitivity of bacteria to different antibiotics was different. Isolated Staphylococcus sp. were highly resistant to antibiotics. Danofloxacin, gentamicin and kanamycin were sensitive whereas amoxicillin/clavulanic acid and ampicillin/sulbactam were moderately sensitive. Most bacteria were resistant to penicillin G and cefaperazon.