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Articles by M.N. Amin
Total Records ( 17 ) for M.N. Amin
  M.M. Rahman , M.N. Amin and R. Ahmed
  In the present study an efficient tissue culture method for high rate of shoot regeneration was developed for Elaeocarpus robustus. Cotyledonary explants with and without petiolar end were cultured in ½ MS medium supplemented with auxin and cytokinin. Explant with the petiolar/proximal end showed a better response than those without it in respect of shoot initiation. Half strength of MS medium supplemented with 1.0 mg l-1 BA+0.5 mg l-1 2,4-D was found to be best for producing organogenic callus. The best shoot proliferation was observed when cotyledon-derived callus was subcultured in medium fortified with a combination of 1.0 mg l-1 BA+0.5 mg l-1 Kn+0.1 mg l-1 NAA. In vitro elongated shoots were rooted with 80% success by treating them ½ in MS medium supplemented with 0.2 mg l-1 IBA and incubated under 30°C in dark for initial one week. Rooted shoots (plantlets) were successfully established into soil and the survival rate of the plantlets was about 55%.
  M.M. Rahman , M.N. Amin , H.S. Jahan and R. Ahmed
  Sprouted rhizome buds were collected from the field grown one year old rhizomes and surface sterilized by treating them with 0.1% HgCl2 for 14 min. The dissected rhizome bud explants (ca.1 cm) of turmeric were cultured in MS medium supplemented with different concentrations and combinations of cytokinin and auxin. The best result towards development of multiple shoot from the cultured explants was obtained when MS medium was supplemented with 2.0 mg l-1 of BA. Rooting experiments with half strength of MS medium and various concentrations of NAA, IBA and IAA revealed that 0.1-1.0 mg l-1 of any auxins was found to be effective for rooting. But root forming performance of IBA was proved to be the best among the three auxins tested. Rooted shoots (plantlets) were gradually acclimatized and successfully established in plastic pot containing garden soil under natural condition. About 70% of the transplanted plantlets survived and were eventually established in the field.
  M.M. Rahman , M.N. Amin , T. Ahamed , M. R. Ali and A. Habib
  Somatic embryogenesis and subsequent plantlets regeneration were achieved in callus cultures established from the leaf base explants of Kaempferia galanga L. Callus induction and somatic embryogenesis at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D+1.0 mg L-1 BA. After transfer on MS medium supplemented with 2.0 mg L-1 BA+0.1 mg L-1 NAA, this callus produced small globular embryos first that later developed into plantlets by further subculturing on the same medium. Plantlets were acclimatized and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 85%.
  F. Begum , M.N. Amin , S. Islam and M.A.K. Azad
  Nodal segments of in vitro germinated seedlings of three pummelo varieties [Var.-1 (pulp is pink colour), Var.-2 (pulp is white colour) and Var.-3 (pulp is red colour)] were cultured on half-strength MS medium for axillary shoot proliferation. A large number of shoot buds were produced when such four weeks old culture were subcultured on half-strength MS medium containing 1.0 mg l¯1 BAP. Roots were induced when the isolated individual shoots were cultured on half-strength MS medium containing 0.1 mg l¯1 each of NAA, IBA or IAA. Cent percent root were observed on half-strength MS medium having 0.1 mg l¯1 NAA. These in vitro grown plantlets were then successfully transferred to outside natural condition through successive phases of acclimatization. About 93% of the regenerated plantlets survived under ex vitro condition.
  M.Z. Karim , M.N. Amin , M.A. Hossain , S. Islam , Faruk Hossin and R. Alam
  Protocol for induction of callus and regeneration response of two sugarcane varieties (Isd-16, Isd-28) was established through callus culture using leaf sheath. Multiple shoot regeneration at various frequencies was observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in the medium containing 3.0 mg l -1 2,4-D with 10% coconut water (CM). The best response in terms of multiple shoot formation was observed that on MS medium supplemented with BAP 1.0 mg l -1 +IBA 0.5 mg l -1. NAA (3.0 mg l -1) was found effective in the production of roots. The variety Isd-16 showed better response than the variety Isd-28 towards shoot multiplication. Seventy percent of the plantlets produced from in vitro culture method survived in the ex vitro condition.
  M.A.K. Azad , M.N. Amin and F. Begum
  The proliferating axillary shoot cultures were established on MS medium supplemented with different concentrations of cytokinins and auxins, using nodal explants from the field grown mature plant of A. vasica. In vitro response of the explants to multiple shoot regeneration varied greatly with the position of the explanting branch on the donor plant. Highest frequencies of shoot formation and maximum number of shoots per explant were obtained on MS medium supplemented with 0.5 mg/l BA. The elongated shoots were rooted successfully on half strength of MS medium with 0.1-0.2 mg/l IBA or without any auxin. The complete plantlets thus regenerated in vitro were successfully transferred to the field.
  M.Z. Karim , M.N. Amin , M.A.K. Azad , F. Begum , M.M. Rahman , M.M. Islam and R. Alam
  Shoot multiplication of Chrysanthemum morifolium was achieved from the nodal and shoot tip explants of mature plant using MS medium with different concentrations and combinations of growth regulators. Maximum frequency of explants produced axillary shoot and the highest number of shoots per explant were obtained when MS medium fortified with 1.0 mg l-1 BAP.The combination BAP+GA3 was found effective result. But Kinetin (Kn) showed low performance for producing multiple shoots. The degree of shoot formation was affected by explant types and the exogenous hormonal regime in the medium.
  M.M. Rahman , M.N. Amin , M.A.K. Azad , F. Begum and M.Z. Karim
  Leaf segments from field grown mature plants of Elaeocarpus robustus Roxb. were used to initiate cultures for inducing callus and subsequent differentiation of shoot buds. Callus production was obtained within seven weeks and adventitious shoot formation was observed after five weeks of incubation on ½ MS medium supplemented with 0.5 mg l-1 BA + 0.1 mg l-1 NAA + 15% CW. Complete plantlets were obtained upon transfer of shoot cuttings on ½ MS with 0.1-2.0 mg l-1 IBA, NAA, IAA or without any auxin. The best and healthy rooting was observed on ½ MS medium supplemented 0.2 mg l-1 IBA. In vitro regenerated plantlets were transferred to potting soil and successfully established under natural condition with about 60% survival.
  F. Begum , M.N. Amin , S. Islam , M.A.K. Azad and M.M. Rehman
  Cotyledon explants from in vitro grown seedlings were cultured on half-strength of MS medium with different growth regulators for in vitro indirect regeneration of shoots. Optimum callus formation, when cotyledon explants were cultured on MS containing 1.0 mg l-1 BAP with 5.0 mg l-1 NAA. After that the calli were used for shoot regeneration by transferring them  half-strength MS medium supplemented with only cytokinin. Maximum percentage of shoot regeneration was obtained on half strength MS medium in the presence of 1.0 mg l-1 BAP from callus in three varieties [Var.-1 (pink colour), Var.-2 (white colour) and Var.-3 (red colour)] of pummelo. For rooting, shoot cuttings were cultured on half strength MS salts with 0.1-1.0 mg l-1 NAA, IBA or IAA. The best and healthy rooting was observed on 0.1 mg l-1 NAA. The plantlets of three varieties were successfully established on soil. About 95% of plantlets survived under ex vitro condition.
  M.M. Rahman , M.N. Amin and S. Ahmad
  Shoot tip and nodal segments from the field grown mature plants of native-olive (Elaeocarpus robustus Roxb.) were used as explants and were cultured on half strength of MS medium supplemented with different types of growth regulators either alone or in combinations. Among the growth regulators and supplements (BA, Kn, NAA and coconut water) that were used in the proliferation medium the best result was observed on 2 MS medium supplemented with 0.5 mgl G 1 BA + 0.5 mgl G 1 Kn + 0.1 mgl G 1 NAA + 15% CW, which promoted multiple shoot bud formation and sufficient shoot elongation. The well-developed shoots were excised to 3-4 cm cuttings and implanted individually on root induction medium. Highest percentage of micro cuttings showed root formation when they were cultured on 2 MS medium containing 0.2 mgl G 1 IBA and incubated under 30° C in dark for initial one week. The in vitro regenerated plants were successfully established in pot holes containing coco-peat on plastic tray and maintained under polythene tents. Survival of the plantlets under ex vitro condition was 60%.
  A. Habib , M.R. Ali , M.N. Amin and M.M. Rahman
  The present study was undertaken with a view to develop a reproducible protocol for in vitro propagation of white mulberry (Morus alba L.). For this purpose, surface sterilization of the explants from field grown mature plants was done with 0.1% HgCl2 with a treatment duration of 5 minutes for shoot tip and that with 6 minutes for nodal segments. Between the two explants used, nodal explant exhibit comparatively better response to axillary shoot proliferation. Analysis of the results obtained from the proliferation experiment proved that effectiveness of cytokinin BA was better than that of Kn with respect to axillary shoot formation. And that MS medium supplemented with 1.0 mg l G 1 BA was found to be best where 100% of the explants proliferated with the axillary shoots having average length of 5.46 " 0.02 cm and a multiplication rate of 7 fold per 4-week. On the other hand, percentage of root induction and number of roots per shoot were largely affected by the concentration of MS medium and type of the auxins used. The highest percentage (100%) of root regeneration was obtained in half MS medium supplemented with 0.5% mg l G 1 IBA.
  M.M. Rahman , M.N. Amin , S. Ahmad and R. Ahmed
  Micropropagation of native-olive (Elaeocarpus robustus Roxb.) has been proved difficult due very slow rooting of the shoot cuttings. The objective of the present investigation was to develop a technique for in vitro fast rooting of the aseptically grown shoots of native-olive. Rooting experiment was conducted on 1/2MS medium with various concentrations and combinations of NAA, IBA and IAA along with or without incubation at higher temperature (30 ° C) in dark condition for initial one week. Root forming performance of IBA was proved to be the best among the three auxins tested. Highest frequency of rooting with maximum number of effective roots was observed on the hormone-free medium in normal growth room condition after treating the microcutting with 0.2 mg L-1 IBA and incubated under 30 ± C in dark for initial one week. Early emergence and fast growth of roots without any malformation were also observed at the same treatment. At this culture condition 85% of the microcuttings produced 5.80 ± 0.15 roots per cutting where average length of roots per cutting was 4.65 ± 0.18 cm. The plantlets originated through the above treatment established themselves under ex vitro condition much quicker than those originated through other treatments.
  M.M. Rahman , M.N. Amin , R. Ahmed , M.A.K. Azad and F. Begum
  Regeneration of multiple shoots via callus induction and organogenesis were achieved in native-olive (Elaeocarpus robustus). Callus induction and shoot buds regeneration were obtained from internode explants of Elaeocarpus robustus on their sufficient medium. The best organic callus was found on modified MS (MMS1) medium supplemented with 0.5 mg L1 BA+0.5 mg L1 2,4-D. Development of adventitious shoots occurred when the calli were subcultured on MMS1 medium supplemented with BA and NAA. Maximum frequency (80%) of calli induced adventitious shoots with highest number of 14.05 ± 1.56 shoots per callus were obtained when the medium was fortified with 1.0 mg L1 BA+0.1 mg L1 NAA. Plantlets developed roots when in vitro developed microcuttings were implanted on modified MS (MMS2) medium with 0.2 mg L1 of IBA. Within six weeks of transfer, 65% rooting was achieved on this medium. Rooted shoots (plantlets) were gradually acclimatized and successfully established under natural condition with about 50% survival rate.
  M.M. Rahman , M.N. Amin , T. Ahamed , S. Ahmad , A. Habib , R. Ahmed , M.B. Ahmed and M.R. Ali
  An efficient protocol has been established for rapid production of plantlets using rhizome tip and lateral bud explants of the field grown plant. The explants were cultured on MS medium with auxins (NAA, IBA and IAA) and cytokinins (BA and Kn). Cent percent of the explants produced two or three shoot buds in each culture when they were cultured on MS medium containing 1.0 mg L -1 BA+0.1 mg L -1 NAA within three weeks of culture. The number of shoots per culture increased gradually when the primary cultures were subcultured in two weeks intervals. Highest number of 20.50±1.80 shoots proliferated in each culture when the explants of initially sprouted shoots were subcultured at three times on the same medium. Microshoots were isolated from the in vitro proliferated cluster of shoots produced roots in 100% cases on modified (MMS2) medium supplemented with 0.2 mg L -1 of IBA. Maximum number of 12.4±1.23 roots per microshoot were recorded on the medium containing 0.2 mg L -1 IBA. The regenerated plantlets were acclimatized and established on the soil with eighty five percent success.
  Shahrear Ahmad , M.N. Amin and M. Ashik Mosaddik
  Phytochemical screening and alkaloid estimation of in vitro and field grown plants of R. serpentina has been investigated. Under the phytochemical screening moisture content (at 45 and 105oC), biomass yield and alkaloid content in vitro and field grown plant materials were determined. At 45oC in vitro plant materials (leaf, root and callus) have higher moisture content compared with field grown (3 years mature plants) plant materials (leaf and root). But opposite result was observed at 105oC. Percent yield of crude plant extract (Biomass yield) of in vitro plant materials was higher than that of field grown plant materials. The percentage of alkaloid for in vitro plant materials was 1.17% in callus, 0.98% in root and 0.49% in leaf, respectively. On the other hand it was found that the field grown plant materials were 0.71% in leaf and 1.43% in root, respectively.
  M.Z. Karim , M.N. Amin , Asaduzzaman , S. Islam , Faruk Hossin and R. Alam
  An efficient protocol was developed for direct regeneration, multiplication and rooting under in vitro conditions of Chrysanthemum. The frequency of multiple shoot regeneration response was 95 and 91%, for nodal segments and shoot tips, respectively when cultured on the medium containing MS +1.0 mg l-1 BAP. Efficient rooting was achieved on half strength of MS+0.2 mg l-1 IBA. In vitro raised plantlets were transferred to potted soil and finally transferred to the field.
  M.A. Rahman , M.N. Amin , M.S. Islam , M.M. Begum and M.A. Uddin
  Air layering on five different times with the help of indole butyric acid (IBA) at 1000, 1500, 2000, 2500, 3000 and 3500 ppm concentration was evaluated in BARI-1 variety of litchi. Five different dates of layering were 15 May, 01, 16 June, 01 and 16 July. It was observed that the number and average length of primary roots and survival of layers were markedly increased where layers were prepared on 01 and 16 June. Layers prepared with IBA at 2500 ppm produced the maximum number (27.14) and length (6.69 cm) of primary roots and obtained the highest percentage (77.66%) of success in the survivability of litchi layers. The best rooting performance and the final survival of air layers in the nursery after 90 days of severing was recorded to be the highest (90%) where layers were prepared on 16 June with IBA at 2500 ppm.
 
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