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Articles by M. Chamani
Total Records ( 5 ) for M. Chamani
  A.R. Seidavi , S.Z. Mirhosseini , M. Shivazad , M. Chamani and A.A. Sadeghi
  In the present study, a multiplex Polymerase Chain Reaction (mPCR)-based assay was developed for the simultaneous identification of Salmonella sp., Escherichia coli and Bifidobacterium sp., that isolated from broiler gastrointestinal in Iran. In order to detect these bacteria species, we were used a set of specific primers that produce different fragment size. Our results showed that all tested gastrointestinal segments were contaminated by these species and therefore the mPCR method is a appropriate method to identify these species directly from poultry gastrointestinal samples. There was no difference in the sensitivity of this method between samples of different gastrointestinal segments. This method has the potential to save considerable time and effort within the laboratory. Our developed mPCR is fast, sensitive, specific and can be used as a reliable choice for routine detection method of these bacteria species in chick gastrointestinal contents.
  T. Tanha , H. Amanlou , M. Chamani , Y. Ebrahimnezhad , R. Salamatdost , N. Maheri and M. Fathi
  The objective of this study was to investigate whether consuming of protected glutamine before and after parturition would affect biomarkers of oxidative stress, dry matter intake and production performance. Thirty six pregnant Holstein dairy cows were assigned into four treatment groups based on their BCS, parity and expected calving date in a completely randomized design. Treatment groups consisted of glutamine supplementation Before and After Calving (BFAF), glutamine supplementation before calving and without glutamine after it (BFAN) without glutamine before and glutamine supplementation after calving and (BNAF) and without glutamine pre and postpartum (BNAN). There were not any significant differences and interaction among treatments in DMI but DMI on days 21 was affected by post partum glutamine feeding. Milk yield was not affected by treatments. The Total Antioxidant Status (TAS) influenced by postpartum glutamine feeding and was most for BFAF and BNAF. The Plasma Glutathione Activity (PGA) was affected by pre and postpartum treatments and the effect of post calving feeding was more clear and there was only an interaction effect on 7 days.
  H.R. Shahbazi , A.A. Sadeghi , H. Fazaeli , G. Raisali , M. Chamani and P. Shawrang
  The effect of electron beam irradiation at doses of 100, 200 and 300 kGy on ruminal dry matter degradation kinetics of wheat straw was investigated. Samples were irradiated by electron beam irradiator under identical conditions of temperature and humidity. Nylon bags of untreated or irradiated wheat straw were suspended in the rumen of three Taleshi steers for up to 96 h and resulting data were fitted to non-linear degradation model to calculate degradation parameters of dry matter. Results show that the washout fractions of dry matter increased linearly (p<0.001), but potentially degradable fraction and degradation rate decreased linearly (p<0.001) by electron beam irradiation. As a consequence, the effective degradability of dry matter increased linearly (p<0.001) with increasing irradiation dose. Electron beam irradiation at doses of 100, 200 and 300 kGy increased the effective degradability of dry matter at rumen outflow rate of 0.05/h by about 7, 15 and 18%, respectively. In the condition of this study, electron beam irradiation at dose of 200 kGy appeared to be a suitable dose for improving dry matter degradability of wheat straw in the rumen.
  A. Toghdory , N. Torbatinejad , R. Kamali , M. Mohajer and M. Chamani
  An experiment was conducted by eight lactating Holstein cows with an average milk production of 32.75 kg day-1 and body weight of 643.6 kg to evaluate the effects of propylene glycol (PG) on productive performance, blood metabolites and nutrients digestibilities. In this experiment a balanced change-over design with four treatments and four periods with 21 days were employed. Treatments included: (1) Control (without PG), (2) 250 g PG/cow/day, (3) 500 g PG/cow/day and (4) 750 g PG/cow/day. Daily milk yield recorded and milk samples were taken during seven and two last days of each period. The results show that dry matter intake, milk yield, fat corrected milk yield, milk compositions were not affected (p>0.05) by different levels of PG. Supplementing diets with 500 and 750 g PG/cow/day, significantly increased plasma glucose (p<0.05) but other blood metabolites such as blood urea nitrogen, triglyceride and cholesterol were not affected (p>0.05) by PG. Apparent digestibility of dry matter and organic matte was not affected (p>0.05) by PG administration. In conclusion, plasma glucose was increased by using 500 and 750 g PG/cow/day (as powder) in the first and mid lactation stage, but the levels of 250 g PG/cow/day did not have any significant effect on dry matter intake, milk yield, milk compositions and other blood metabolites.
  T. Tanha , H. Amanlou , M. Chamani , Y. Ebrahimnezhad , R. Salamatdost , N. Maheri , M. Fathi and M. Abozar
  The objective of this study was to investigate whether consuming of Protected Glutamine (PG) before parturition in close up period would affect biomarkers of oxidative stress, immune system and Haptoglobin (HP) and Serum Amyloid A (SAA). About 36 pregnant Holstein dairy cows were assigned into two treatment groups based on their BCS and expected calving date in at student examination. Treatment groups consisted of glutamine supplementation 100 g day-1 per cow before calving (F), glutamine did not supplementation before calving (N). There were not any significantdifferences among treatments in DMI and BCS on 21, 14 and 7 days before parturation. There were not significant differences in the Total Antioxidant Status (TAS), Haptoglobin (HP), Serum Amyloid A (SAA), No Esterified Fatty Acids (NEFA) and blood and immune cells. The plasma Glutathione Peroxidase activity (GPX) was significant difference between two group and it seems that supplementation diets with glutamine on the close up period can enhanceplasma Glutathione Peroxidase activity (GPX).
 
 
 
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