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by
L.N. Mathuram |
Total Records (
2 ) for
L.N. Mathuram |
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P. Ravi Kumar
,
M. Kanniappan
,
L.N. Mathuram
,
S. Selvasubramanian
and
P. Sriram
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Hexaconazole is a triazole fungicide used in crop protection. Triazoles are one of the promising groups of fungicides that act by inhibiting the biosynthesis of ergosterol, an essential component of fungal cell membrane, via inhibition of cytochrome P450 dependent enzyme lanosterol 14α-demethylase. The present study is aimed to screen hexaconazole for its in vivo clastogenic potential in Wistar strain rats. The doses of hexaconazole selected in the present study were 182, 365 and 730 mg kg-1 for male rats and 506, 1012 and 2024 mg kg-1 for females. These doses were corresponding to 1/12th, 1/6th and 1/3rd, respectively of earlier reported oral LD50 values which were 2189 mg kg-1 in males and 6071 mg kg-1 in females. The in vivo clastogenic potential of hexaconazole was studied employing bone marrow chromosomal aberrations assay and micronucleus test following single exposure and multiple exposures to the drug. Results of bone marrow chromosomal aberrations assay indicated that hexaconazole in the tested doses is incapable of producing any structural or numerical aberrations in both male and female rats. Analysis of the bone marrow smears of the slides for micronucleated polychromatic erythrocytes revealed no significant increase in their number in hexaconazole treated rats. Multiple exposures also could not enhance their incidence significantly. These observations confirmed the results of chromosomal aberrations assay in this study where hexaconazole failed to produce and any aberrations. It further appears from the present study that hexaconazole had no effect on spindle formation during cell division. |
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P. Ravi Kumar
,
M. Kanniappan
,
L.N. Mathuram
,
S. Selvasubramanian
,
B. Murali Manohar
and
P. Sriram
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Hexaconazole is a triazole fungicide extensively used in agricultural
and horticultural practices. The fungicidal action of triazoles is mainly attributed
to their ability to inhibit cytochrome P450 dependent enzyme lanosterol 14 α-demethylase.
Lanosterol 14 α-demethylase is necessary for the conversion of lanosterol
to ergosterol which is an essential component of fungal cell membrane. Cytochromes
P450 are ubiquitous heme containing proteins that are found through out the
plant and animal kingdom and play a crucial role in the synthesis of steroidal
hormones in mammals. Any interference in the synthesis of sex steroidal hormones
will obviously affect the reproductive performance. Thus, hexaconazole was studied
for its effect on histological architecture of male and female reproductive
system in Wistar rats. Male rats were administered hexaconazole per os daily
at 0.0, 27.5, 55.0 and 110.0 my kg-1 body weight for 30 and
60 days. Female rats were also given hexaconazole at the same dose level but
for 30 and 73 days. |
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