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| Articles
by
K. Nasirujjaman |
Total Records (
4 ) for
K. Nasirujjaman |
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M. Salah Uddin
,
K. Nasirujjaman
,
S. Zaman
and
M.A. Reza
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The present study was conducted to establish a protocol for rapid multiplication of shoots from different in vitro grown explants viz. shoot tip, nodal segment and cotyledonary node of Peltophorum pterocarpum. All the explants were cultured on MS media containing different concentrations and combinations of BAP, KIN and NAA. Among different hormonal concentrations and combinations, KIN+NAA showed the best result and cotyledonary node explants gave best shoot multiplication. The highest number (4.50 %) of multiple shoots was observed in MS media containing 2.0 mg L-1 KIN+0.5 mg L-1 NAA. In some cases, BAP also showed better result. The regenerated shoots were transferred on MS media having IBA for adventitious root initiation. Then the plantlets with elongated shoots and root system were considered ready to transfer to soil. |
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M.R. Islam
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S. Zaman
and
K. Nasirujjaman
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In the present study plant regeneration techniques of Aegle marmelos from nodal explant have been developed. For inducing callus, nodal segments derived from in vitro grown shoots were cultured on Murashige and Skoog (MS) medium supplemented with different compositions of phytohormones. The maximum callus formation was observed on MS medium having 0.3 mg L-1 BA+2.0 mg L-1 2,4-D. In all cases, callus proliferation began at the cut ends of the explants. Optimum organogenesis and plantlet formation occurred when the calli were subcultured on medium fortified with 2.0 mg L-1 l BA+0.1 mg L-1 NAA. Callus-derived shoots produced roots when transferred to half strength of MS medium without any growth regulators. The rooted plantlets were successfully transplanted to soil. |
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M. Salah Uddin
,
K. Nasirujjaman
,
M.M. Rahman
and
M.A. Reza
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The present study was conducted to induce callus from different in vitro grown seedling explants viz., cotyledon, nodal segment and leaf segment and to develop shoot buds from callus. At first, all the explants were cultured on MS medium supplement with different concentrations and combinations of BAP, KIN, NAA and 2,4-D. Among all the explants, cotyledon showed best performance for callus induction. For this explant, the highest callusing (93.33%) and the highest fresh weight of callus (1.38 g) was observed in MS+2.0 mg L 1 BAP+0.5 mgL 1NAA media composition. Among all the concentrations and combinations, higher concentration of cytokinin (BAP) with lower concentration of auxin (NAA) was proved to be the best for callus induction. Calli, which were obtained from cotyledon and leaf segment in MS+2.0 mg L 1 BAP+0.5 mg L 1 NAA were subcultured in MS media having different concentrations and combinations of BAP and KIN with NAA. It was found that MS medium supplemented with KIN-NAA combination was the best for development of shoot buds from calli. |
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K. Nasirujjaman
,
M. Salah Uddin
,
S. Zaman
and
M.A. Reza
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An ideal micropropagation method of turmeric (Curcuma longa Linn.) has
been developed using rhizome bud explants. Woody Plant Medium supplemented with
different concentrations of BAP alone or in combination with different
concentrations of NAA produced varying degree of multiple shoots. A
supplementation of 4.0 mg L 1 BAP + 1.0 mg L 1 NAA gave
the best result. In this case, 95% of the inoculated explants induced multiple
shoots within 8-10 days of inoculation and the average number of shoots per
explant was 6.70. Rooting was spontaneous in almost all the treatments. Most of
the regenerated shoots were successfully transferred to soil under field
conditions. |
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