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The objective of the present study was to estimate the seroprevalence Toxoplasma gondii infection in people working on pig farms in Longyan city, the People's Republic of China. A total of 212 human serum samples were collected from different farms and assayed for T. gondii antibodies (IgG) by Enzyme-Linked Immunosorbent Serologic Assay (ELISA). The results showed that the over all seroprevalence of T. gondii IgG in people working on pig farms with T. gondii IgG was 12.26% (26/212) with the farm managers having the highest prevalence (23.53%, 4/17) which was followed by the pig breeders (11.54%, 21/182) and the farmers (7.69%, 1/13). The present study demonstrated that the prevalence of T. gondii infection in people working on pig farms is high. Therefore, people working in pig farms need to be informed of the infection risk with T. gondii. Integrated control strategies and measures should be implemented to prevent and control T. gondii infection in both humans and animals in these pig farms.