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Articles by Arzu Tas Caputcu
Total Records ( 2 ) for Arzu Tas Caputcu
  Tolga Akkoc , Ali Cihan Taskin , Arzu Tas Caputcu , Sezen Arat and Haydar Bagis
  The aim of this study was to compare Solid Surface Vitrification (SSV) technique and classic vitrification technique in in vitro produced 8 days old bovine blastocysts. Cryopreservation of mammalian embryo has great importance for genetic resources conservation, embryo transfer, veterinary and clinical reproductive biotechnology and animal assisted reproductive technologies. Immature oocytes were maturated then fertilized with frozen-thawed bull semen and cultured until blastocyst stage in commercial sequential culture medium for 8 days. Blastocysts were vitrified in two different groups as SSV and classic vitrification and non-vitrified blastocysts were used as control group. After vitrification, vitrified blastocysts were warmed and cultured for 1 day. For this aim, blastocyst viability rate and median cell number were investigated. The blastocyst viability rate that vitrified by classic vitrification (34.8%) were found to be lower than those vitrified by SSV (82.6%) and control group blastocysts (100%). However, median cell numbers of vitrified-warmed blastocysts were found higher in SSV (124) than classic vitrification (104). Median cell number of control group was detected as 213. As a result, blastocyst viability rate and median cell number in SSV group was higher than classic vitrification group, there was a significant difference between SSV and classic vitrification group (p<0.05).
  Ali Cihan Taskin , Tolga Akkoc , Arzu Tas Caputcu , Sezen Arat and Haydar Bagis
  The researchers investigated the effect of blastocyst development and quality of blastomere aspiration techniques on eight-cell mouse embryos. The results clearly indicate that the in vitro development of biopsied mouse embryos depended on the suitable aspiration method. This method related to pre-implantation genetic diagnosis for the genesis of animal models for animal disorders from embryos. In this study, female CB6 F1(Balb/cXC57bl/j) hybrid mice were superovulated with hormones and superovulated females were sacrified approximately 68 h after hCG administration. About eight-cell embryos were recovered from oviducts of sacrified mouse in M2 medium. Before biopsy, all embryos were incubated to decrease cell to cell contacts to microdrops of Ca2+/Mg2 + free QAM HTF (3 mg mL-1 BSA Fraction V) with HEPES for 90 min at 37°C. A single blastomere of eight-cell embryos were aspirated by the aspiration pippetes under inverted microscope (4X). After biyopsy, embryos were cultured in SAGE medium supplemented with 5% CO2, 5% O2 and 90% air at 37°C for up to expanded blastocyst stage. After culture, 267 biopsied embryos out of 234 (88.32±7.5%) expanded blastocysts developed from the biopsy group and total cell number mean 67.8±17.2% were recorded in the experiment group and 126 non-biopsied embryos out of 118 (94.4±7.78%) expanded blastocysts developed from control embryos and total cell number mean 70±15.4% was recorded in the control group. In the present study, there was no difference in blastocysts developmental rates at post biopsy group and control group (p = 0.05). In conclusion, the biopsy the method described here is an optimal method of blastomere aspiration on in vivo mouse embryos.
 
 
 
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