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Ali A. Al-Salamah |
Total Records (
4 ) for
Ali A. Al-Salamah |
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A. Maripandi
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Ali A. Al-Salamah
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A study was conducted to determine the prevalence, antibiotics resistant and plasmid patterns of Salmonella enteritidis on chicken meat samples. A total of 578 chicken meat samples were examined over a period of 2 years from different retail outlets of a residential area of Namakkal City, South India. S.enteritidis prevalence was recorded 92 of 578 (15.91%). Seasonal variations in the prevalence pattern were identified with, a higher prevalence during monsoon months (19.68%) followed by post-monsoon (17.61%) and premonsoon. Present finding revealed that S. enteritidis isolates recovered from retail raw meats are resistant to more than one antibiotics, including those commonly used antibiotics in poultry feed were erythromycin, ampicillin, kanamycin, cephalothin and tetracyclin. All the isolates exhibited Multiple Antibiotic Resistance (MAR) and more than one plasmid. The plasmid size ranged between 0.43 and 115 MDa. Prevalence of multiple antimicrobial resistance among these strains suggesting possible prior selection by use of antimicrobials in meat production. |
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A. Maripandi
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Ali A. Al-Salamah
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Problem statement: Salmonella infection is a serious medical and veterinary problem world-wide and causes great concern in the human health and food safety. Salmonella enteritidis outer membrane proteins and lipopolysaccharide play important role in the virulence and immunological properties of bacteria. Prophylaxis of enterobacterial infection has proven difficult to achieve. Vaccination is an effective tool for the prevention of Salmonella infection. Approach: Therefore, a study was conducted on S. enteritidis isolates from chicken meat samples for Outer Membrane Proteins (OMPs) and Lipopolysaccharide (LPS) profile were analyzed by SDS-PAGE electrophoresis and immunodominent antigen was detected by western blot analysis. Results: S. enteritidis with different OMPs bands were exhibited with molecular weight ranging from 5-90 kDa. The major outer membrane protein profiles of all S. enteritidis isolates were homogenous with different expression in intensity of protein was observed. LPS of different S. enteritidis isolates exhibited doublet band was observed and identified as R type of strains. The immunoblotting results at 14.4 and 24 KDa proteins were good immunogen. Conclusion: The 14.4 and 24 KDa proteins were immune response protein. This proteins can use for vaccine development. |
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Maripandi Arjunan
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Ali A. Al-Salamah
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M. Amuthan
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Problem statement: Urinary Tract Infections (UTIs) in human, rural environment cause significant morbidity due to insanitary condition, lack of knowledge of personal hygiene, lack of patient’s compliance and economic burden. In our study, the bacterial etiologies and the resistance patterns found in human with serious UTIs and selecting optimal antimicrobial therapy. Approach: A total of 105 patients first morning mid stream urine samples, culture was done by the calibrated loop technique delivering 0.001 mL of urine plated on Cystine-Lactose-Electrolyte Deficient (CLED) agar, MacConkey agar and Blood agar medium (Hi Media, India) for isolation of uropathogens. All pathogens were analyzed for drug susceptibility by disk diffusion method. Results: More than 50% of them were confirmed to have UTI bacteriological positive. Women and man especially in the age group of 20-29 had higher incidence of bacterial infection. A total of 58 isolates seven different uropathogens were identified among the Escherichia coli (31%) was dominant pathogens followed by Citrobacter spp., (20%) and Pseudomonas aeruginosa (17.24%). In all uropathogens antibiotics susceptibility the more active agents were norfloxacin (87.93%), ciprofloxacin (70.68%), gentamicin (70.68%), nalidixic acid (68.96%), nitrofurantoine (60.34%) and tetracycline (60.34%) and less active in ampicillin (8.62%), rifampicin (37.93%) and carbenicillin (41.37%). Conclusion: Among commonly used antimicrobial agents for the treatment of UTI, there is a trend towards increasing resistance to ampicillin and a persistently low resistance rate to norfloxacin, ciprofloxacin and gentamicin should be consider for treatment. |
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Abdelnasser S.S. Ibrahim
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Ali A. Al-Salamah
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Media and cultivation conditions were investigated to optimize alkaline protease production by alkaliphilic Bacillus halodurans. This includes different carbon, nitrogen and metals sources in addition to different pH, incubation temperature and aeration level. The specific enzyme activity was increased by about 48.8 fold by optimizing different nutrient sources and cultivation conditions. The maximum specific enzyme activity was obtained in a medium containing 15 g L-1 lactose as the carbon source, 6 g L-1 soybean as the nitrogen source and a 5 mM mixture of Mg, Mn and Ca as trace elements, fermentation for 48 h at 37°C and agitation at 200 rpm. This study indicated the significance of nutrient source and cultivation conditions on the alkaline enzyme production by Bacillus halodurans. |
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