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Here we report the results of a 2-step study of the effects induced by near infrared low intensity laser radiation on bovines affected by subacute mastitis. In the first step, the correlation between Somatic Cells Count (SCC), Lactose and Chloride ion foremilk content in healthy and mastitic quarters of Italian Friesian cows, were studied and compared with the literature data. In the second step, the effects of the Near Infrared Low Intensity Laser Radiation (NIRLILR) on the cow affected by subacute mastitis, were evaluated in terms of 2 average quarter population parameters, the "average Somatic Cell Count" (SCC) and the "average Restoration Factors" (RF). It was revealed that the NIRLILR radiation treatment, in the experimental conditions adopted, caused an effective beneficial response of the cows against the mastitis.