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Articles by Z.G. Yuan
Total Records ( 5 ) for Z.G. Yuan
  Q.J. Zhuang , H.J. Zhang , R.Q. Lin , Z.G. Yuan , X.J. Liang , X.W. Qin , W.J. Pu and X.Q. Zhu
  The present study aimed to examine whether Mycoplasma Haemofelis (MHF) and Candidatus Mycoplasma Haemominutum (CMH) occur in cats in Mainland China. Genomic DNA was extracted from 87 cat blood samples collected from Guangzhou, China and they were examined by conventional Polymerase Chain Reaction (PCR) assay to detect and distinguish infection of MHF and CMH. The total infection rate of cats with MHF and CMH was 42.5% (37/87), with one female cat being infected with MHF, while 41.4% (36/87) of the infections were due to CMH and none of the cats was positive for concurrent infection with both MHF and CMH. Sequencing of representative amplicons confirmed the results of PCR amplifications. This result, for the first time, demonstrated the existence of MHF and CMH in cats in mainland China.
  Y.S. Mahmmod , F.A. El-Balkemy , Z.G. Yuan , M.F. El-Mekkawy , A.M. Monazie and X.Q. Zhu
  The study evaluated the usefulness of specific PCR assays for the diagnosis of tropicaltheileriosis caused by Theileria annulata in Egypt using Microscopic Examination (ME) for comparison. Blood samples from 258 animals comprising both sick and apparently healthy cattle and water buffaloes were examined for the presence of Theileria infection by ME using Giemsa-stained blood smears and the prevalence was 18.6% (48/258). To evaluate the usefulness of PCR assays for the identification Theileria sp. and T. annulata, 30 bovine blood samples selected from the 258 animals were tested by both PCR assays and ME and ME identified 9/30 (30%), whereas the PCR assay for Theileria sp. was more sensitive which identified 21/30 (70%). Of these 21 Theileria positive samples, 15 were identified as infected with T. annulata by specific PCR assay for T. annulata. This study demonstrated that specific PCR assays are more sensitive and accurate for the clinical diagnosis of tropical theileriosis.
  G.H. Peng , Z.G. Yuan , D.H. Zhou , X.H. He , C. Yan , C.C. Yin , Y. He , R.Q. Lin , H.Q. Song and X.Q. Zhu
  Toxoplasma gondii infects nearly one third of the total population of the world, as well as warm blooded animals causing serious public health problems and economic losses in the world. Micronemes plays a key role in the invasion process of T. gondii which are used for host cell recognition, binding and motility. In this research, the researchers examined sequence variation in the microneme protein 4 (MIC4) gene sequences of 12 T. gondii isolates and reference strains from different hosts and geographical locations, then constructed the DNA vaccine expressing MIC 4 of T. gondii evaluated its immune response induced in Kunming mice. The results demonstrated that sequence variation in MIC 4 among different T. gondii isolates was low, which is a useful feature as a vaccine candidate. Immunization of mice with pVAX-MIC4 induced strong immune responses in mice as shown by significant lymphocyte proliferation, cytokine production and antibody responses as well as increased survival time of the immunized mice after challenge with tachyzoites of the virulent T. gondii RH strain, demonstrating that T. gondii MIC4 is a potential vaccine candidate against toxoplasmosis.
  H.K. Yan , H.Q. Song , Y. Zhou , D. Ren , D.H. Zhou , M.J. Xu , R.Q. Lin , X.Q. Zhu and Z.G. Yuan
  Perforin-like Protein 1 (PLP1) plays an important role in the invasion process of T. gondii. In this study, we examined sequence variation in the PLP1 gene among six T. gondii strains from Guangzhou and Panyu in Guangdong, Suhe in Henan, Huzhu in Qinghai provinces of China, France and the USA, representing different genotypes. The PLP1 gene was amplified from the >6 strains by Polymerase Chain Reaction (PCR) and the amplicons were cloned and sequenced. The length of all of the PLP1 sequences was 3453 bp, consistent with that available in GenBank (EF 102772.2). In total, there were 42 (1.22%) variable nucleotide positions among the six PLP1 gene sequences and the 22 of which represented transversions. The A+T contents of the sequences was 48.3~48.9%. Intra-specific nucleotide variation was related mainly to changes at the 2nd and 3rd codon positions while fewer changes were detected at the 1st codon position. These results demonstrated that sequence variation in PLP1 gene among the six T. gondii strains was low and the PLP1 gene may not be an appropriate marker for the studies of genetic variation among T. gondii strains.
  F. Chen , J. Li , H. Sugiyama , Y.B. Weng , F.C. Zou , R.Q. Lin , Z.G. Yuan , H.Q. Song , X.Q. Zhu and G.H. Zhao
  In the present study, a portion of the 18S and 28S ribosomal DNA (rDNA) sequences of 35 Schistosoma japonicum isolates representing three geographical strains from mainland China, the Philippines and Japan were amplified and compared and phylogenetic relationships were also reconstructed by Unweighted Pair-Group Method with Arithmetic averages (UPGMA) using combined 18S and 28S rDNA sequences as well as the corresponding sequences of other species belonging to the Schistosoma genus available in the public database. The results indicated that the partial 18S and 28S rDNA sequences of all S. japonicum isolates were 745 and 618 bp, respectively and displayed low genetic variation among S. japonicum strains and isolates. Phylogenetic analysis revealed that the combined 18S and 28S rDNA sequences were not able to distinguish S. japonicum isolates from three geographical origins but provided an effective molecular marker for the inter-species phylogenetic analysis and differential identification of different Schistosoma species.
 
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