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This study is to demonstrated that microsatellites markers
developed for Tor tambroides can be used to amplify microsatellite
loci in other family. It is assumed that microsatellite loci are more
conserved for aquatic species compared to terrestrial ones due to aquatic
environments are less mutagenic than terrestrial ones. Development of
microsatellites still requires investment of time and resources. Thus
using loci already developed in a related species may provide a cost-effective
alternative to microsatellite isolation and development in a species of
interest in present study, Probarbus jullieni. In this study we
investigated the possibility of the conservation of microsatellite flanking
regions among different species. Nine pairs of SSR primers, five gave
very strong banding profile (SYK1, SYK 2, SYK 5 SYK 8 and SYK 9) which
could be used for population studies by using the nested protocol. Results
showed that SYK 2 and SYK 9 flanked the same (CA)n repeats
and thus are highly conserved in a different species. The products of
the SYK 5, 8 and 1 primer pairs showed differences in the microsatellite
regions which they flanked in Probarbus jullieni when compared
to those of the source species, Tor tambroides. The mean observed
heterozygosity levels for all the primers ranged 0.23-0.81. The primers
are all polymorphic with the mean number of alleles from 2-5.
