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Umblachery cattle was assessed genetically using 25 microsatellite markers, as recommended by FAO. The number of alleles was ranging from 2 to 6 with a mean of 4.0±0.11. The mean number of effective alleles were 2.91±0.09. The allele sizes were ranging from 94 to 300 bp with the frequency distribution of 0.0111 to 0.9375. The estimated heterozygosity value was high 0.6139±0.02 and the PIC was 0.5625±0.03 and the loci screened were polymorphic and overall mean FIS value (-0.0487) suggested the excess of heterozygosity in the population.