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by
R. Heidari |
Total Records (
2 ) for
R. Heidari |
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R. Heidari
,
M. Khayami
and
T. Farboodnia
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In this research we examined the effect of 0.25, 0.5 and 1 mM Pb(No3)2 with and without 0.5 mM EDTA in pH 4 and 6 on the growth and Pb accumulation in the root and shoot of four day old seedlings of Zea mays at the controlled condition in the period of 72 h. At the end of treatment we determined the length and dry weight of the root and Pb content in the root and shoot of the seedlings. In the second test we considered specially the effect of 0.25, 0.5, 1, 1.5 and 2 mM EDTA with 0.5 mM Pb(No3)2 on the seedlings of Zea mays at the same condition. After 72 h we determined the amount of Pb by Atomic Absorption and EDTA by HPLC and water content of shoot. We concluded (from the results of two tests) that: Pb absorption has grown up parallel to its concentration in growth solution and has inhibited root growth and biomass significantly. Pb taking up in pH 4 was higher than pH 6 and EDTA enhanced Pb accumulation in shoot. But water content of shoot decreased at the concentrations more than 0.5 mM EDTA in growth solution. Maximum level of EDTA accumulated in shoot of plants was at the concentration of 1.5 mM EDTA in the culture, but the highest level of Pb and the least water loss of shoot was at the equimolar Pb and EDTA (0.5 mM Pb with 0.5 mM ETDA). It may concluded that EDTA is taken up by plants, accumulated in shoots in the form of Pb-EDTA or protonated form and enhanced Pb accumulation in shoots of seedlings. So it can be used for phytoextraction of Pb and other metals not only by accumulators but also by tolerant plants such as Zea mays. But if EDTA is used for supplying plant micronutrients, its concentration should be minimized and its damage to plants should be clearly considered. |
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A. Afaghi
,
S. Zare
,
R. Heidari
,
Y. Asadpoor
and
R. Malekzadeh Viayeh
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The objective of this study was to evaluate the possible effects of CuSO4 exposure on variations of glucose and cortisol levels in Cyprinus carpio. Three replicates of 6 fish were subjected to two sub-lethal concentrations of CuSO4 (0.16 and 0.53 mg L-1) for 14 and 21 days. Blood samples were isolated from the fish following the exposure, to measure the levels of cortisol and glucose compared to the control group. The results showed significant increases (p<0.05) in cortisol levels for both fish groups after 14 days of exposure, whereas, the levels of blood cortisol in both groups did not differ from that of control when the fish subjected to copper sulfate for 21 days. We found significant increases (p<0.05) in the levels of blood glucose of two groups of fish after 14 days of exposure to two doses of CuSO4, as well as significant decrease in the blood glucose of both groups exposed for 21 days. In the later treatment, the rate of decrease in group II (exposed to 0.53 mg L-1 CuSO4) was higher than that of group I (exposed to 0.16 mg L-1 CuSO4) (p = 0.001 compared to p = 0.032). Our findings attest that exposing to waterborne copper would affect the levels of both cortisol and glucose, as indicators of stress response in Cyprinus carpio. |
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