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A rapid worldwide expansion of mobile telephones raises questions regarding possible effects of the emitted radiofrequencies on the health of the consumers. The mobile phone system (GSM-900) works in the range of 890-960 MHZ in the electromagnetic spectrum. The present study was performed to investigate the effect of mobile phone radiation on auditory system of rabbit. The auditory brainstem response (ABR), was studied before and after using a mobile phone in the hearing of rabbit. After measuring of click and tone burst at different frequencies (500, 1000, 2000, 4000 and 8000 HZ) with two intensities of 70 and 100 dB, the animals were exposured to electromagnetic waves from a simulator of mobile phone one week exposure and 16-19 h rest. The ABR tests were shown that the latency time of wave V (ms) have some changes in the frequencies of the experiments. The latency time of wave V (ms) at the frequencies of 500 and 1000 HZ was almost unchanged, but at the frequencies of 2000, 4000 and 8000 HZ were decreased at the end of second week of exposure. Statistical analysis have not any significant changes between time latency of wave in pre and post exposures.