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Articles by Min Li
Total Records ( 9 ) for Min Li
  Min Li , Yuanping Wang , Nianli Zou , Sanjie Cao , Xintian Wen and Yong Huang
  Interleukin-6 (IL-6) is a multifunctional cytokine which has a wide range of activity in animals and has an impact on almost all immune system cells. To produce specific antibody against chicken IL-6 and detect its activity, its complete Open Reading Frame (ORF) sequence was amplified and inserted into the eukaryotic expression vector pcDNA3.1 (+) to get recombinant eukaryotic plasmid pcDNA-ChIL-6 and BALB/c mouse were immunized with this plasmid to produce polyclonal antibody against Chicken IL-6. Then the mature protein coding genes of chicken IL-6 was cloned and inserted into the prokaryotic expression vector pET-32a (+) to get recombinant prokaryotic plasmid pET-ChIL-6, the expression of this fusion protein was successfully induced by Isopropy 1-β-D-thiogalactoside (IPTG) and the recombinant protein was further purified by NTA His•Bind column. The purified recombinant protein could react positively with polyclonal antibody against pcDNA-ChIL-6 in Western-blot and indirect ELISA.
  Binglian Bai , Haitao Wang , Peng Zhang , Songnan Qu , Fan Li , Zhixin Yu , Beihong Long and Min Li
  The synthesis and mesomorphic behaviour are reported of a new series of dimers containing 4-nitrobenzohydrazide and azobenzene groups as the mesogenic units. These non-symmetric liquid crystal dimers are found to exhibit a monolayer smectic A phase (SmA1). Lateral hydrogen bonding and strong dipole-dipole interactions are shown to be the major driving forces for the formation of the SmA1 phase. The present study indicates that the intermolecular interactions and thus the mesophase morphology of the liquid crystal dimers can be controlled by the appropriate selection of the molecular fragments capable of forming H-bonds.
  Haitao Wang , Fenglong Zhang , Binglian Bai , Peng Zhang , Jianhua Shi , Dingyi Yu , Yunfeng Zhao , Yue Wang and Min Li
  A series of hexacatenar liquid crystals containing the 1,3,4-oxadiazole group as rigid core, i.e. 1,4-bis[(3,4,5-trialkoxyphenyl)-1,3,4-oxadiazolyl]- benzene (P-P-oxd-n), were designed and synthesised. Based on a detailed study of their thermotropic phase behaviour and mesophase structures, it was revealed that columnar phases are generated in these materials. Furthermore, combination of experimental and calculated results enabled a proposal for the molecular packing in the mesophase. The photoluminescent properties of these materials were examined using P-P-oxd-8 as an example. A strong blue light emission (λmax = 456 nm) was observed in P-P-oxd-8 and a higher quantum yield was obtained in dilute chloroform solution.
  Haitao Wang , Renfan Shao , Chenhui Zhu , Binglian Bai , Chengbo Gong , Peng Zhang , Fan Li , Min Li and Noel A. Clark
  Dimeric hydrazide derivatives with long terminal alkoxy chains, i.e. α,ω-bis[N-(4-hexadecyloxybenzoyl)-N’-(benzoyl-4’-oxy)hydrazine]alkanes (C16. n. C16, C16 indicates the terminal hexadecyloxy chain; n = 3, 5, 6, 10, indicates the number of the carbon atoms in the spacer) were synthesised. The liquid crystalline properties were investigated by differential scanning calorimetry, polarising optical microscopy and X-ray diffraction. The dimers with shorter spacer (C16. n. C16, n = 3, 5, 6) exhibit enantiotropic smectic phases, whereas C16.10.C16 with the longest spacer is non-mesomorphic. Furthermore, the smectic structure is parity dependent, i.e. C16.3.C16 and C16.5.C16 with odd spacer exhibit a monolayer smectic A phase, whereas C16.6.C16 with even spacer exhibits a monolayer smectic C phase, in which the incompatibility between the terminal chains, mesogenic groups and the spacers is considered to be the driving force. The results are compared with those for the terminally nitro-substituted series, α,ω-bis[N-(4-nitrobenzoyl)-N’-(benzoyl-4’-oxy)hydrazine]alkanes (Nn), which exhibit an intercalated smectic A phase. Temperature-dependent IR spectroscopic analysis on these two kind of dimers suggests that intermolecular hydrogen bonding, as well as the dipole-dipole interaction arising from the strong polar substituents (-NO2) synergistically drives the intercalated structure of Nn. Although hydrogen bonding exists in the monolayer smectic phase of C16. n. C16, microphase segregation is in favour of the monolayer smectic structure, preventing the formation of an intercalated structure.
  Peng Zhang , Songnan Qu , Binglian Bai , Haitao Wang , Xia Ran , Chengxiao Zhao and Min Li
  ymmetrical four-chained (tetracatenar) di-hydrazine derivatives, namely oxalyl N',N'-bis(3,4-dialkoxybenzoyl)-hydrazide (BFH-n, n = 4, 6, 8, 10), were synthesised. Investigations on the liquid crystalline properties by differential scanning calorimetry (DSC), wide-angle X-ray diffraction (WAXD) and polarising optical microscopy (POM) showed that the di-hydrazine derivatives exhibited columnar mesophases and the symmetry of the mesophase changes from rectangular to hexagonal on increasing the temperature. The rectangular columnar mesophases of BFH-n (n = 6, 8, 10) remained stable down to 10°C during cooling and the subsequent recrystallisation from the Colr phase of BFH-n (n = 6, 8, 10) was observed on the second heating runs. Furthermore, the average number of molecules packing in a column slice was estimated to be three, based on their X-ray diffraction results. Intermolecular hydrogen bonding between -C=O and -N-H groups in crystalline and liquid crystalline phases was confirmed.
  Zhen-Yu Wang , Yuan-Zheng Xin , Dong-Mei Gao , Min Li , J. Morgan and Bao-Shan Xing
  Five different sites with a soluble salt gradient of 3.0–17.7 g kg−1 dry soil from the coast to the inland were selected, and the microbial population size, activity and diversity in the rhizospheres of five common plant species and the adjacent bulk soils (non-rhizosphere) were compared in a degraded wetland of the Yellow River Delta, Shandong Province, China to study the effects of soil environment (salinity, seasonality, depth, and rhizosphere) on microbial communities and the wetland's ecological function, thus providing basic data for the bioremediation of degraded wetlands. There was a significant negative linear relationship between the salinity and the total number of microorganisms, overall microbial activity, or culturable microbial diversity. Salinity adversely affected the microbial community, and higher salinity levels resulted in smaller and less active microbial communities. Seasonal changes were observed in microbial activity but did not occur in the size and diversity. The microbial size, activity and diversity decreased with increasing soil depth. The size, activity and diversity of culturable microorganisms increased in the rhizospheres. All rhizospheres had positive effects on the microbial communities, and common seepweed had the highest rhizosphere effect. Three halophilic bacteria (Pseudomonas mendocina, Burkholderia glumae, and Acinetobacter johnsonii) were separated through BIOLOG identification, and common seepweed could be recommended for bioremediation of degraded wetlands in the Yellow River Delta.
  Silvia G. Bompadre , Min Li and Tzyh-Chang Hwang
  Cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel gated by ATP binding and hydrolysis at its nucleotide binding domains (NBD). The NBDs dimerize in a head-to-tail configuration, forming two ATP binding pockets (ABP) with the ATP molecules buried at the dimer interface. Previous studies have indicated that ABP2, formed by the Walker A and B motifs of NBD2 and the signature sequence of NBD1, is the site critical for the ATP-dependent opening of CFTR. The G551D mutation in ABP2, the third most common cystic fibrosis-associated mutation, abolishes ATP-dependent gating, resulting in an open probability that is ∼100-fold lower than that of wild-type channels. Interestingly, we found that the ATP analog N6-(2-phenylethyl)-ATP (P-ATP) increases G551D currents mainly by increasing the open time of the channel. This effect is reduced when P-ATP is applied together with ATP, suggesting a competition between ATP and P-ATP for a common binding site. Introducing mutations that lower the nucleotide binding affinity at ABP2 did not alter significantly the effects of P-ATP on G551D-CFTR, whereas an equivalent mutation at ABP1 (consisting of the Walker A and B motifs of NBD1 and the signature sequence of NBD2) dramatically decreased the potency of P-ATP, indicating that ABP1 is the site where P-ATP binds to increase the activity of G551D-CFTR. These results substantiate the idea that nucleotide binding at ABP1 stabilizes the open channel conformation. Our observation that P-ATP enhances the G551D activity by binding at ABP1 implicates that ABP1 can potentially be a target for drugs to bind and increase the channel activity.
  Zhaobing Gao , Qiaojie Xiong , Haiyan Sun and Min Li
  Chemical openers for KCNQ potassium channels are useful probes both for understanding channel gating and for developing therapeutics. The five KCNQ isoforms (KCNQ1 to KCNQ5, or Kv7.1 to Kv7.5) are differentially localized. Therefore, the molecular specificity of chemical openers is an important subject of investigation. Native KCNQ1 normally exists in complex with auxiliary subunits known as KCNE. In cardiac myocytes, the KCNQ1-KCNE1 (IsK or minK) channel is thought to underlie the IKs current, a component critical for membrane repolarization during cardiac action potential. Hence, the molecular and pharmacological differences between KCNQ1 and KCNQ1-KCNE1 channels have been important topics. Zinc pyrithione (ZnPy) is a newly identified KCNQ channel opener, which potently activates KCNQ2, KCNQ4, and KCNQ5. However, the ZnPy effects on cardiac KCNQ1 potassium channels remain largely unknown. Here we show that ZnPy effectively augments the KCNQ1 current, exhibiting an increase in current amplitude, reduction of inactivation, and slowing of both activation and deactivation. Some of these are reminiscent of effects by KCNE1. In addition, neither the heteromultimeric KCNQ1-KCNE1 channels nor native IKs current displayed any sensitivity to ZnPy, indicating that the static occupancy by a KCNE subunit desensitizes the reversible effects by a chemical opener. Site-directed mutagenesis of KCNQ1 reveals that residues critical for the potentiation effects by either ZnPy or KCNE are clustered together in the S6 region overlapping with the critical gating determinants. Thus, the convergence of potentiation effects and molecular determinants critical for both an auxiliary subunit and a chemical opener argue for a mechanistic overlap in causing potentiation.
  Min Li , Changgong Li , Yi-hsin Liu , Yiming Xing , Lingyan Hu , Zea Borok , Kenny Y.-C. Kwong and Parviz Minoo
  In vertebrates, Sonic hedgehog (Shh) and transforming growth factor-β (TGF-β) signaling pathways occur in an overlapping manner in many morphogenetic processes. In vitro data indicate that the two pathways may interact. Whether such interactions occur during embryonic development remains unknown. Using embryonic lung morphogenesis as a model, we generated transgenic mice in which exon 2 of the TβRII gene, which encodes the type II TGF-β receptor, was deleted via a mesodermal-specific Cre. Mesodermal-specific deletion of TβRII (TβRIIΔ/Δ) resulted in embryonic lethality. The lungs showed abnormalities in both number and shape of cartilage in trachea and bronchi. In the lung parenchyma, where epithelial-mesenchymal interactions are critical for normal development, deletion of mesenchymal TβRII caused abnormalities in epithelial morphogenesis. Failure in normal epithelial branching morphogenesis in the TβRIIΔ/Δ lungs caused cystic airway malformations. Interruption of the TβRII locus in the lung mesenchyme increased mRNA for Patched and Gli-1, two downstream targets of Shh signaling, without alterations in Shh ligand levels produced in the epithelium. Therefore, we conclude that TβRII-mediated signaling in the lung mesenchyme modulates transduction of Shh signaling that originates from the epithelium. To our knowledge, this is the first in vivo evidence for a reciprocal and novel mode of cross-communication between Shh and TGF-β pathways during embryonic development.
 
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