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| Articles
by
M.Z. Karim |
Total Records (
7 ) for
M.Z. Karim |
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M.Z. Karim
,
S. Yokota
,
M.M. Rahman
,
J. Eizawa
,
Y. Saito
,
M.A.K. Azad
,
F. Ishiguri
,
K. Iizuka
and
N. Yoshizawa
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A highly reproducible in vitro shoot regeneration system in Aralia elata Seem. using root explants was developed in this investigation. Multiple shoots were induced in vitro directly from root explants through adventitious shoot bud regeneration. The ability of root explants to produce shoot buds depended on the supplementation of Plant Growth Regulators (PGRs). Maximum multiplication of shoots (18 shoots per explant) was achieved in a Broad-leaved Tree (BT) medium supplemented with 1.00 μM 6-benzyl aminopurine (BAP). The best and healthiest rooting was observed in a BT medium supplemented with 2.00 μM α-naphthaleneacetic acid (NAA). Regenerated plants were successfully acclimatized under ex vitro condition. |
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M.Z. Karim
,
M.N. Amin
,
M.A. Hossain
,
S. Islam
,
Faruk Hossin
and
R. Alam
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Protocol for induction of callus and regeneration response of two sugarcane varieties (Isd-16, Isd-28) was established through callus culture using leaf sheath. Multiple shoot regeneration at various frequencies was observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in the medium containing 3.0 mg l -1 2,4-D with 10% coconut water (CM). The best response in terms of multiple shoot formation was observed that on MS medium supplemented with BAP 1.0 mg l -1 +IBA 0.5 mg l -1. NAA (3.0 mg l -1) was found effective in the production of roots. The variety Isd-16 showed better response than the variety Isd-28 towards shoot multiplication. Seventy percent of the plantlets produced from in vitro culture method survived in the ex vitro condition.
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M.Z. Karim
,
M.N. Amin
,
M.A.K. Azad
,
F. Begum
,
M.M. Rahman
,
M.M. Islam
and
R. Alam
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Shoot multiplication of
Chrysanthemum morifolium was achieved
from the nodal and shoot tip explants of mature plant using MS medium
with different concentrations and combinations of growth regulators.
Maximum frequency of explants produced axillary shoot and the highest
number of shoots per explant were obtained when MS medium fortified
with 1.0 mg l-1 BAP.The combination BAP+GA3 was
found effective result. But Kinetin (Kn) showed low performance for
producing multiple shoots. The degree of shoot formation was affected
by explant types and the exogenous hormonal regime in the medium. |
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M.M. Rahman
,
M.N. Amin
,
M.A.K. Azad
,
F. Begum
and
M.Z. Karim
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Leaf segments from field
grown mature plants of Elaeocarpus
robustus Roxb. were used to initiate cultures for inducing callus
and subsequent differentiation of shoot buds. Callus production was
obtained within seven weeks and adventitious shoot formation was observed
after five weeks of incubation on ½ MS medium supplemented
with 0.5 mg l-1 BA + 0.1 mg l-1 NAA + 15% CW.
Complete plantlets were obtained upon transfer of shoot cuttings on
½ MS with 0.1-2.0 mg l-1 IBA, NAA, IAA or without
any auxin. The best and healthy rooting was observed on ½
MS medium supplemented 0.2 mg l-1 IBA. In vitro regenerated plantlets were
transferred to potting soil and successfully established under natural
condition with about 60% survival. |
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R. Baksha
,
R. Alam
,
M.Z. Karim
,
Sk. A. Mannan
,
B.P. Podder
and
A.B.M.M. Rahman
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Microshoots were cultured on MS medium supplemented with different combinations
of auxin, sucrose and pH level for in vitro rooting of callus induced sugarcane
(Saccharum officinarum) variety namely Isd 31. Among the types and concentrations of
auxin used the best rooting in all the microshoots was obtained with half strength (major
salts) MS basal medium supplemented with NAA at 5.0 mg l‾1, sucrose at 50 g l‾1 and
pH level 5.7 were the optimum conditions for rooting when microshoots were incubated
25+ 2°C under 16 h photo period regime. The regenerated plantlets were successfully
transferred to soil and the percentage of survivability under ex vitro conditions was 75. |
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M.Z. Karim
,
R. Alam
,
R. Baksha
,
S.K. Paul
,
M.A. Hossain
and
A.B.M. Mafizur Rahman
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Leaf sheath were used as explants to induce callus on modified MS media supplemented with 2, 4-D as growth regulators. Different concentrations of BAP, IBA, NAA and IAA were used to regenerate shoots and a concentration of 1.0 mg/l BAP and 0.5mg/l IBA was found superior in the optimum production of multiple shoots. NAA (5mg/l) showed best performance in the production of roots. The plantlets were successfully transferred to soil with 70 percent survivability. |
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M.Z. Karim
,
M.N. Amin
,
Asaduzzaman
,
S. Islam
,
Faruk Hossin
and
R. Alam
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An efficient protocol was developed for direct regeneration, multiplication and rooting under in vitro conditions of Chrysanthemum. The frequency of multiple shoot regeneration response was 95 and 91%, for nodal segments and shoot tips, respectively when cultured on the medium containing MS +1.0 mg l-1 BAP. Efficient rooting was achieved on half strength of MS+0.2 mg l-1 IBA. In vitro raised plantlets were transferred to potted soil and finally transferred to the field. |
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