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by
M.M. Hossain |
Total Records (
8 ) for
M.M. Hossain |
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M.M. Rahman
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M.M. Hossain
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Optimum plant density of soybean varies with geographic location and variety. The present study was undertaken with a view to optimize plant density of two soybean varieties using equidistant planting patter to obtain higher yield. The experiments were conducted in three consecutive seasons viz., Rabi 2004-05, Kharif 2005 and Rabi 2005-06 at Mymensingh, Bangladesh with two soybean varieties G-2 and PB-1 and six plant densities viz., 20, 40, 60, 80, 100 and 120 plants m-2 established using an equidistant planting pattern of 22.4x22.4 cm, 15.8x15.8 cm, 12.9x12.9 cm, 10.0x 10.0 cm and 9.1x 9.1 cm, respectively. Seed yield increased with increase of plant density up to 80 to 100 plants m-2 depending on variety and season. The increase in plant density decreased yield components such as number of pods plant-1, seeds pod-1 and 100-seed weight as well as seed yield plant-1. The soybean seed yield was positively correlated with total dry matter and leaf area index. The present study concludes that the highest soybean yield could be possible with a plant density of 80-100 plants m-2 depending upon variety, season and related agronomic management options. |
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S.M. Raquibul Hasan
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M.M. Hossain
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R. Akter
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M. Jamila
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M.E.H. Mazumder
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M.A. Alam
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A. Faruque
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S. Rana
and
S. Rahman
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Analgesic potential of the pet ether (PECB), chloroform (CCB), n-butanol (NBCB) and hydromethanolic (HMCB) fractions of the aerial parts of Commelina benghalensis Linn. was evaluated for centrally acting analgesic property using hotplate and tail immersion method and peripheral pharmacological actions using acetic acid-induced writhing test to scientifically validate some of the folkloric and ethnomedical uses of the plant. All fractions, at the dose of 200 and 400 mg kg-1 b.wt., displayed significant analgesic action in a dose dependent manner in the tested models. In acetic acid-induced writhing test, all extracts exhibited significant (p<0.05) reduction of writhing response in a dose dependent manner; the response decreased in the order Diclofenac-Na (76.16%) > CCB2 (68.8%) > NBCB2 (61.9%) > HMCB2 (52.8%) > PECB2 (48.0%). In hotplate and tail immersion method, all fractions caused a significant (p<0.0-0.001) increase in latency time and the results are comparable to the standard drug Nalbuphine. These results suggest significant analgesic potential of C. benghalensis and thereby justify its traditional uses in various types of pain. |
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M. Muktaruzzaman
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M.G. Haider
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A.K.M. Ahmed
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K.J. Alam
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M.M. Rahman
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M.B. Khatun
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M.H. Rahman
and
M.M. Hossain
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Salmonella infections are major problems for the poultry farming in Bangladesh. The cultural method to identify avian Salmonella infections is laborious and expensive, thus a rapid, sensitive and cost-effective method for the diagnosis of salmonellosis is anticipated. In the present investigation Salmonella pullorum organisms was obtained from the Department of Pathology and it was characterized by culture, biochemical tests and PCR. A neotetrazolium stained Salmonella pullorum antigen was prepared from local isolate of Salmonella pullorum. The protein concentration of stained antigen was measured by BSA standard curve. Prepared antigen was diluted in 2 fold dilution and minimum 72.5 μg/μl antigen concentration showed the positive reaction. Different preservatives (0.5% phenolized saline, 0.5% formalized saline and 0.09% sodium azide) were used to maintain the shelf life of prepared antigen. All the preservatives showed the similar results up to six months. Slide agglutination tests were carried out with un-diluted and diluted anti-sera having known ELISA titre and end point agglutination titre was determined. Serum titre 13942-21362 gave positive result to 2-5 fold dilution of serum and serum titre 412-771 showed negative result. Different groups of antigens were developed while antigen group-1 (48 h bacterial culture treated with 24 h in neotetrazolium and 2 h in thiomersal) gave the striking positive result. As Group-1 antigen exhibited highest protein concentration (1240 μg/μl) and gave the best result with positive sera, so it was selected for field trial. The seroprevalence of Salmonella infection was 44.39% in a particular poultry farm. The stained antigen was then stored at 4oC. As all used preservatives revealed similar trend of results, so it may be recommend that 0.5% phenolized saline as preservative because it is cost effective. In the present study, the slide agglutination test was found easy, sensitive, reliable, cost and time effective and needed very small amount of antigen, sera and as well as accessories. Salmonella pullorum antigen from a local isolate was successfully developed which could be used to screen the Salmonella infection in the poultry flocks at the farm premises. It may also be used to determine the antibody titer of the vaccinated flocks. |
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A.B.M. Shahinuzzaman
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A.K. Saha
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A.C. Mazumder
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S. Das
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M.A. Sufian
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M.A. Baki
and
M.M. Hossain
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The research work was conducted to study the sequential pathological changes of Pullorum Disease (PD) and immunohistochemical detection of its causal agent (Salmonella pullorum) in various tissues of experimentally infected chicks. Total 40 day old (D0) chicks were divided into experimental and control groups. The chicks were infected at day 15 (D15) of age by oral route with 1 ml of 2 x 108 CFU of S. pullorum. Chicks were sacrificed at day 1 (D1), day 3 (D3), day 5 (D5), day 7 (D7) and day 9 (D9) of Post Infection (PI) and observed the remarkable gross lesions in liver, lung, heart and cecum. Grossly, liver found fragile (40%) at D7 and D9. Cheesy materials in cecum (20%) showed at D9. The highest reisolation of S. pullorum demonstrated in cecum (68%). Histopathologically, nodular lesion in liver developed at D7 (20%) and D9 (40%). Hepatitis from D1 (20%) and continued upto D9 (60%). Pneumonia and bronchopneumonia along with inflammatory cells in lung were observed at D1 (20%) that continued upto D9 (80%). Spleen showed depletion of lymphocytes at D7 (40%) and D9 (60%). Typhlitis in cecum noticed at D5 (20%) and remained up to at D9 (40%). Congestion and hemorrhage was common in organs at early times and gradually reduced both in grossly and histopathologically thereafter. Immunohistochemistry revealed S. pullorum bacteria in the cytoplasm of hepatocytes of liver, in the cytoplasm of epithelia of cecum (D3, D7) and in the cytoplasm of epithelia of crop (D3). |
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M.A.H. Beg
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M.A. Baqui
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N.R. Sarker
and
M.M. Hossain
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A total of five-hundred and fifty (550) day-old Cobb-500 broiler chicks were used for a period of six weeks to study the effect of different stocking densities and feeding regime on the performance of broiler chicken. The treatments under stocking densities were D1 (8 bird/m2), D2 (10 bird/m2), D3 (12 bird/m2) and D4 (14 bird/m2). The form of feeds under feeding regime was F1 (mash), F2 (crumble) and F3 (pellet) feeds. The study revealed that the lower stocking density D1 consumed significantly (p<0.05) the highest amount of feed and in terms of feed form F2 group consumed significantly (p<0.05) the highest amount of crumble feed and among the interaction between ‘density x feed’ D2F2 consumed significantly (p<0.05) the highest amount of feed. The average live weight of birds under stocking density D3 was significantly (p<0.05) higher compared to other density groups; there was no significant difference (p<0.05) among the mash, crumble and pellet groups. The interaction between D3F2 was achieved significantly (p<0.05) highest live weight than other treatment groups. Irrespective of feed type, FCR value was significantly better in D4 and D3 (p<0.05) compared to D2 and D1. Similarly irrespective stocking density, crumble feed performed better FCR compared to others and the interaction group D3F2 showed significantly better (p<0.05) FCR compared to other combinations. The birds under lower stocking density D1 consumed significantly (p<0.05) the highest amount of water; but the form of feeds (F1, F2 and F3) had no significant (p<0.05) effect on water intake and the water intake was significantly higher (p<0.05) in D1F1 compared to other treatments. No significant difference (p<0.05) in mortality was found among different stocking densities, feeding regime and different interaction groups. The Benefit Cost Ratio (BCR)/m2 under D3 was significantly (p<0.05) higher compared to others; the F1, F2 and F3 feeds had no significant effect on BCR and D3F2 group was significantly (p<0.05) profitable than other combinations. The dressing % of stocking density D1 and D2 were significantly (p<0.05) higher compared to D3 and D4. No significant difference (p<0.05) in dressing % was found among F1, F2 and F3 feeds, similar results were observed in different carcass parts for stocking density and feed types. Significantly higher (p<0.05) abdominal fat deposition were found higher in D3 and D4. Lower stocking density showed higher dressing percent. The stocking density and feeding regimes had no significant effect on different carcass parts of broiler except higher abdominal fat deposition was found in higher stocking density groups. Finally, it can be concluded that during summer the stocking density 12 bird/m2 fed on crumble feed may be profitable for commercial broiler production up to six weeks in Bangladesh condition. |
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M.M. Hossain
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M.N. Hassan
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A.AKM. Nowsad
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Sylhet-Sunamgonj region is one of the most important freshwater fish producing areas of Bangladesh. A huge amount of fish is produced in these regions to support local consumption and also export to other countries. The caught fish are not adequately taken care off during harvest and post-harvest handling. So, a serious loss in wet fish occurs. The present study has determined the extent of quality loss in fish value chain in Sylhet-Sunamgonj regions with a view to minimize such losses. Extent of loss in wet fish quality at the stakeholder level during transportation like fish farmer, arotder (commission agent), wholesaler and retailer was assessed. Two big landing centers, one big wholesale markets and one retail market of Sylhet-Sunamgonj region were studied. Data were collected through questionnaire interview along with traveling throughout the distribution channel. A sensory method was used to assess the quality of wet fish. Insufficient icing, mishandling of fish, non-insulated carrying container and lack of awareness were found to be the reasons for the quick quality loss of wet fish in these regions. |
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M.M. Hossain
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K. Kawai
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S. Oshima
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Japanese eel Anguilla japonica were immunized with inactivated Edwardsiella tarda bacterin preparations {formalin-killed cells, FKC (0.4%), formalin with heat-killed cells, FHKC (0.1% and 70°C for 10 min), heat-killed cells, HKC (70°C for 15 min), potassium chloride-killed cells, KKC (0.6%), tannic acid-killed cells, TKC (0.9%), citric acid-killed cells, CAKC (0.9%), pressure-killed cells, PKC (600 psi for 5 min) and electric current-killed cells, ECKC (100 mA at 12 v DC for 5 sec) via intraperitoneal injection in order to develop adequate inactivating method. Immune parameters in the immunized eel were measured to compare responses to different bacterins. Generally, eel rose agglutinating antibody titer in the serum within 2 week and the maximum titer occurred at 6 weeks post immunization. Elevated and significantly higher titer was produced with the PKC of E. tarda than other bacterin preparations. An Enzyme Linked Immunosorbent Assay (ELISA), to determine specific anti-E. tarda antibody in the serum, also showed significantly higher antibody titer with PKC than the other antigen preparations. Bacteriostatic assay with serum and live E. tarda indicated significantly higher activity in the PKC-immunized fish. Immunization with PKC also showed the increased level of phagocytosis. PKC-inactivated vaccine at an immunization dose of 106 cells/fish induced high protection against experimental infection. Coincident with higher immune parameters and protection in the fish immunized with the PKC bacterin strongly suggested that pressure-killing is an effective inactivating method to develop an effective vaccine against edwardsiellosis. |
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K.U. Ahmed
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M.M. Rahman
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M.Z. Alam
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M.M. Hossain
and
M.G. Miah
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The study of seasonal influence on incidence of trunk borer infestation was undertaken during 2010 at Kapasia upazila under district of Gazipur, Bangladesh. The borer was found in orchard from June to September with a peak emergence in mid July. The larval population of Jackfruit trunk borer is the destructive pest stage, which evokes concern in jackfruit growing areas of Bangladesh. The highest percentage of infestation was in July (7.33%) followed by June and August (6.00%). The cumulative infestation over the year in the study area was 35.33% in October. The lowest infestation was observed in February (0.67%) whereas no activity was found during November to January. The incidence of infestation of trunk borer was influenced by temperature, rainfall and relative humidity due to seasonal variations and their contribution of the regression (R2) were 63, 65 and 31%, respectively. Five independent weather factors in stepwise regression equation pooled responsible for 67.4% of the total variance. Stepwise regression showed that maximum temperature was the most important to influence 35.3% and the influence was lowest (2.1%) in case of average rainfall. |
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