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by
M.I.A. Karim |
Total Records (
4 ) for
M.I.A. Karim |
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S. Abd-Aziz
,
L.G.A. Ong
,
M.A. Hassan
and
M.I.A. Karim
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The aims of this study are to optimised the process
parameters involved for submerged fermentation of mannanase by Aspergillus
niger FTC5003 using palm kernel cake (PKC) as carbon source. The parameters
investigated include temperature, agitation speed, inoculum size and PKC
concentration. The submerged fermentation was carried out for 10 days
by using palm kernel cake as the sole carbon source with the addition
of nitrogen source. Palm kernel cake can be considered as a suitable carbon
source for the enzyme production due to it mainly consist of mannan and
galactomannan, which is hemicellulose. The mannanase enzyme production
using the optimised fermentation condition, which was conducted at agitation
speed of 200 rpm, temperature 35 °C, 1x104 spores mL-1
of inoculum size and 2% of palm kernel cake with productivity of 13.00
U/mL/day. |
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N. Nadir
,
M. Mel
,
M.I.A. Karim
and
R.M. Yunus
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The two-step enzymatic hydrolysis of sweet sorghum and cassava were performed by commercially available α-amylase and glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae were studied. For both sweet sorghum and cassava, the hydrolysis and fermentation were done in a 2 L stirred tank bioreactor, B-Braun fermenter, by using the same conditions. The amount of glucose obtained after hydrolysis process was greater in sweet sorghum compared to cassava, which are 50.07 and 40.00 g L-1, respectively. Also, sweet sorghum gave higher ethanol concentration than cassava at the 64 h of fermentation process, which are 40.11 and 34.07 g L-1, respectively. |
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M. Mel
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M.I.A. Karim
and
H.M. Salleh
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This study is focused on the evaluation of the volumetric mass transfer coefficient (kLa) of E. coli fermentation producing β-glucuronidase enzyme in different bioreactor operating conditions. The operating conditions used were according to the Taguchi’s method design using three factors of fermentation viz., temperature, agitation speed and air flow rate at two concentration levels. From the four experiments conducted, experimental run number two with agitation of 300 rpm, airflow rate of 2 vvm and temperature 41°C generate the highest Optical Density (OD 660 nm) of 2.313 for cell count and also having an equivalent kLa value of 0.0281 min-1 or 1.686 h-1. This condition was found to be an effective point for upscaling in bigger bioreactor for E. coli fermentation to produce the enzyme. |
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Z. Shahrim
,
V. Sabaratnam
,
N.A.A. Rahman
,
S. Abd-Aziz
,
M.A. Hassan
and
M.I.A. Karim
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Trichoderma sp. KUPM0001 showed good growth during solid substrate fermentation (SSF) of sago pith residue known as hampas, supplemented with 10% (v/w) of mineral salts solution containing 0.5% (w/v) (83.3 mM) urea as nitrogen source and an initial moisture content of 80% (v/w). Mycelium suspension of 10% (v/w) density was used as initial inoculum and SSF was carried out at 25±2°C in static condition over a period of 120 h. The parameters optimized included the initial moisture content of the substrate, mineral salts solution, urea concentration, inoculum density, incubation temperature and incubation time. Without optimized condition, the maximum reducing sugar obtained was 24 mg mL-1 compared to 46 mg mL-1 substrate during optimized SSF after 96 h incubation. The optimum parameters obtained were 80% (v/w) of initial moisture; 10% (v/w) of inoculums size; 1.0% of urea in 20% (w/v) of mineral solution and incubated at 30±2°C. The enzyme activities using optimized condition gave maximum α-amylase, glucoamylase, carboxymethyl cellulase, filter paperase and β-glucosidase of 3.19, 2.22, 1.66, 1.11 and 1.48 U mL-1, respectively. |
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