Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the
coverage of the most important and influential journals
to meet the needs of the global scientific community.
This study characterized and investigated optimality of Generalized gradient vector flow active contours as a suitable boundary mapping technique for Chromosome spread images having variability in shape and size. Boundary mapping using generalized gradient vector flow active contours is done on chromosome spread images and it is found that a unique set of parameter values for the technique is required for boundary mapping every chromosome image. Characterization studies have shown that an optimal range of values exists for each parameter within which good boundary mapping results can be obtained for various chromosomes in similar class of images. Statistical testing validates the experimental results.