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The chemical compositions of fishes vary and are closely related to feed intake, migratory swimming and sexual changes in connection with spawning. The nutritive composition of many marine, estuarine and fresh water fishes were determined and have been implemented in fish consumption. Nutritive composition of Dussumieria acuta and Sardinella brachysoma were not yet determined and the present study was dealt with these fishes. Fatty acids, Vitamins (fat soluble and water soluble) and Minerals were quantified. Palmitic acid showed its higher level in both species (5.78 and 3.89%) in D. acuta and S. brachysoma, respectively). Water-soluble vitamins were higher in D. acuta and fat-soluble vitamins showed higher percentage availability in S. brachysoma. Calcium was the predominant mineral in both fishes and can be consumed by humans for bone strength. The finding has proved with strong evidence that both the fishes undertaken for the present study are rich in most of the nutrients essential for proper health maintenance of humans.