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Three hundred adult healthy earthworms (udrillus euginae) ranging length and weight from 14–29cm (mean 21.5±6.8cm) to 1.6–3.0g (mean 2.55±1.1g), respectively, were cultured in three different substrate for 70days. The substrates were soil alone, (control T-1), cow (Bos taurus) dung and water hyacinth (ichhornia crassipes) (1:1 T-2), and partially dried neem (Azadirachta indica) leaves with kitchen waste (1:1 T-3). The earthworms were grown in these substrates in a wooden chamber (0.8m×0.5m×0.2m) and these were subjected to monitoring for various growth patterns (length, biomass, number) at every fortnight (five observations) interval. The observations showed that T-2 was the best among the three treatments used. There was significant difference (P<0.05) between the mean values of growth efficiency in food conversion among the treatments. However, there was no significant difference (P>0.05) between the mean values of survival rate of earthworm among different treatments.