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by
B.K. Sil |
Total Records (
2 ) for
B.K. Sil |
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M. Mehedi
,
K.M. Hossain
,
M.J.F.A. Taimur
,
B.K. Sil
and
M.R. Islam
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The research work was undertaken to prepare haemagglutination antigen of NDV on vero cell line as an alternative to the traditional chicken embryonated egg. Newcastle disease virus can grow within different animal cell line. Vero cell is an established cell line whose source is African green monkey`s kidney. The anchorage-dependent vero cells were first subcultured in Eagle`s minimum essential medium to form semi-confluent monolayer. This monolayer was then infected by collected passage 3 (P3) adapted NDV and maintained up to passage 7 (P7). The antigen was collected from this adapted NDV. Then the NDV antigen was assayed and tested for its purity by tissue culture infective dose (TCID50) assay and haemagglutination test respectively. The titre of NDV was 104.1 TCID50. HA result showed NDV antigen agglutinate chicken red blood cells up to 1600 dilution, which is moderately higher titre than HA titre found for NDV, propagated in chicken embryos. |
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P.K. Biswas
,
B.K. Sil
,
R. Faruque
,
S. Ahmed
,
D. Biswas
and
S. Chowdhury
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A disease outbreak was investigated in a commercial broiler parent stock farm. The outbreak began at the mid of May 2000 and continued more than one month. During the period mortality was recorded around 4 % in the affected shed, flock strength of which was 5000 birds. During post mortem examination the pericardial sac was found engorged with straw colour pericardial fluid. Numerous subcapsular petechial and ecchymotic haemorrhages were seen in liver. No bacterial growth was found after 48 hours of incubation on Muller-Hinton and MacConkey agar surfaces inoculated from the pathological sample. But poor viral growth was recorded in chick embryo fibroblast cell culture propagated inoculums making from heart and liver. Harvested virus using as antigen source from the growth was found reacted positively in agar gel precipitation test with reference conventional adenovirus polyvalent antiserum. Two of the four randomly collected serum samples of the affected flock at day 20 of the outbreak were also found reacting positively with the known reference antigen produced by the same institute. Not having the type specific antiserum the present study failed to elucidate the stereotype (s) that involved in the said outbreak. Nevertheless, the study seems to be the first report of adenoviral induced hydropericardium-hepatitis syndrome or leechi heart disease affecting chicken in Bangladesh. |
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