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by
A.K. Verma |
Total Records (
6 ) for
A.K. Verma |
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A.K. Verma
,
V. Lakshmanan
,
Arun K. Das
,
S.K. Mendiratta
and
A.S.R. Anjaneyulu
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In the present study, physico-chemical and functional
properties of buffalo head meat; heart meat and buffalo skeletal meat
were estimated and compared. Moisture content of buffalo heart meat (78.42%)
and head meat (76.94%) was significantly (p<0.05) higher than buffalo
skeletal meat (75.85%). Buffalo heart meat had significantly lower protein
content (15.49%) than head meat (19.25%) and skeletal meat (19.84%). Fat
and ash content of buffalo skeletal meat, head meat and heart meat did
not differ significantly among themselves. pH of buffalo head meat (6.41)
was significantly higher than skeletal meat (5.85) and heart meat (5.80).
Salt extractable protein of head meat (12.02%) was significantly (p<0.05)
higher than skeletal meat (8.25%) and heart meat (8.52%). Heart meat had
significantly (p<0.05) lower water holding capacity than skeletal and
head meat. Shear force value and emulsifying capacity of heart meat were
significantly (p<0.05) lower than skeletal and head meat. There was
a significant difference in total pigment content between head (398.82
ppm), heart (338.98 ppm) and skeletal meat (243.89 ppm). |
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Rajesh Kumar
,
A.K. Verma
,
Amit Kumar
,
Mukesh Srivastava
and
H.P. Lal
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With the increasing trends of pet ownership the chances of campylobacteriosis are also increasing as these pets are kept in close visicinity of owners. The prevalence and antimicrobial sensitivity profiles of Campylobacter isolates from faeces of dogs attended in veterinary practice at Teaching Veterinary Clinical Complex, Mathura, India. During the period of investigation (October 2009 to April 2010), 100 rectal swabs from dogs were collected and transported to the laboratory for further investigations. Bacteriological examination revealed 51.00% prevalence rate of Campylobacter isolates in dogs faecal samples. The disc-diffusion method was used to know the susceptibility of all the 51 Campylobacter isolates against 10 commonly used antimicrobials in pet animal practice. High rates of resistance were observed to erythromycin (90.20%), tetracycline (88.23%), ampi-cloxacillin (88.23%), ciprofloxacin (80.39%), enrofloxacin (68.63%) and aymoxycillin-clavulanic acid (19.61%). All the Campylobacter isolates were susceptible to amikacin, levofloxacin and streptomycin. Erythromycin and ciprofloxacin are drugs for treatment of human campylobacteriosis. The high resistance rate to these drugs among Campylobacter isolates from dog faeces is of public health significance as dogs are supposed to be the main source of infection in human beings. |
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Rajesh Kumar
,
A.K. Verma
,
Amit Kumar
,
Mukesh Srivastava
and
H.P. Lal
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Campylobacteriosis is one of the leading causes of gastroenteritis in humans and various researches suggested that owning a pet is a risk factor for the disease. To determine the prevalence and risk indicators for Campylobacter sp. infecting dogs attending veterinary practice at TVCC, DUVASU, Mathura, 100 dogs with and without the clinical symptoms of diarrhoea were examined and the prevalence of Campylobacter sp. was 51.0%. Breed wise prevalence showed that nondescript (73.68%) dogs were more likely to carry Campylobacter sp. A significant difference in isolation rates was observed between younger and older dogs: 56.58% of the younger dogs (≤ 1 year) were positive, compared with 33.33% of adult dogs (> 1 year) (p<0.01) as seen at the veterinary University, Mathura, India. Dogs sharing a household with another dog, dogs that had not received antibiotic treatment in the previous months and the age of the dog were significant indicators of Campylobacter carriage. Recent diarrhoea or vomiting in dogs with Campylobacter, breed, sex or vaccination status were not statistically significant. The high prevalence of Campylobacter in puppies supports the hypothesis that dogs, particularly young ones shed Campylobacter spp., which can be of impact for public health. |
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S. Shamshad Ahmad
,
Kafil Akhtar
,
A.K. Verma
,
Amjad Zia Mallik
and
Shahid Ali Siddiqui
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The present study was planned to explore the influence of total peripheral lymphocyte count in cancer patients and to ascertain its prognostic significance. 100 cases were divided into 2 groups: test group comprised of 75 cancerous cases and control group of 25 cases who did not suffer from any problem known to alter the immune status. After a detailed clinical history and examination, routine investigations and certain specific investigations like ultra-sonography, intravenous pyelography, computed tomography, fine needle aspiration cytology and histopathology were performed. Total Leukocyte Count (TLC) using Turks fluid was performed along with differential count from peripheral smear and Total Peripheral Lymphocyte Count (TPLC) was calculated by the formula: TPLC = TLC x percentage of lymphocytes in differential count. Majority of the malignant cases belonged to the fifth decade. Histologically, carcinoma was most frequent, 92.0% cases, followed by sarcoma, 6.6% and melanoma, 1.3% cases. The mean TPLC in the control group was 2759.12±955.7 and it was significantly depressed in all the malignant cases. In breast carcinoma, TPLC was found to decrease with disease progression and was stage dependent. TPLC shows promise as a diagnostic and prognostic indicator of malignancy. |
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K. Dhama
,
S. Rajagunalan
,
S. Chakraborty
,
A.K. Verma
,
A. Kumar
,
R. Tiwari
and
S. Kapoor
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The term food borne diseases or food-borne illnesses or more commonly food poisoning are used to denote gastrointestinal complications that occur following recent consumption of a particular food or drink. Millions of people suffer worldwide every year and the situation is quiet grave in developing nations creating social and economic strain. The food borne pathogens include various bacteria viz., Salmonella, Campylobacter, Escherichia coli, Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus, Arcobacter, Clostridium perfringens, Cl. botulinum and Bacillus cereus and helminths viz., Taenia. They also include protozoa viz., Trichinella, Sarcocystis, Toxoplasma gondii and Cryptosporidium parvum. The zoonotic potential and the ability to elaborate toxins by many of the microbes causing fatal intoxication are sufficient to understand the seriousness of the situation. The viral agents being host specific their transmission to humans through food of animal origin is not yet confirmed although these animal viruses are similar to that of viruses infecting human. Food-borne bacteria; protozoa and helminthes have complex distribution pattern in the environment and inside the host system. This along with complexity of the maintenance chain and life cycle (of parasites) has made it difficult for epidemiologist and diagnostician to undertake any immediate safety measures against them. Serological and molecular diagnostic tests viz. ELISA, Latex agglutination test, Lateral flow assays, Immunomagnetic separation assays, molecular assays viz. Polymerase Chain Reaction (PCR), multiplex PCR, immuno-PCR, Realtime PCR, Random Amplified Polymorphic DNA (RAPD)-PCR, DNA microarrays and probes are widely used. Along with these LAMP assays, Capillary Electrophoresis-Single Strand Confirmation polymorphism (CE-SSCP); Flow cytometry, FISH, Biosensors, Direct epifluorescent filter technique, nanotechnology based methods and sophisticated tools (ultrasonography, magnetic resonance imaging and chlonangio-pancreatography) have aided in the diagnosis greatly. Most of the food-borne illnesses are self-limiting but in many instances antibiotics are recommended. With the increased drug resistance however use of chicken immunoglobulin, bacteriophage therapy, probiotics and herbs are gaining much importance these days. Adoption of proper prevention and control measures (including cooking procedures; hygiene, strict adherence to HACCP principles, public awareness and disease surveillance and monitoring) are the need of hour. All these have been discussed vividly in this review to help epidemiologists, diagnosticians, clinicians and above all common people so as to enable them avoid negligence regarding such serious issue. |
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