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The analysis of soil glucose level and amylase activity in crude oil treated soil following remediation with Poultry Manure (PM) and Sawdust (SD) were studied for four weeks using standard bioremediation techniques. The aim of this research was to analyse the effect of sawdust and poultry manure on the bioremediation of crude oil contaminated soil. Amylase activity was determined by the Richard and Norbert method while glucose was determined by the Barham and Trinder method of analysis respectively. The aim of this study was to determine the role sawdust and poultry manure play in the process of bioremediation of crude oil contaminated soil. The result showed a statistically significant decrease in soil glucose level after bioremediation (p<0.05). The introduction of sawdust, led to increase in soil glucose level on day 14 (0.09±0.222, 0.07±0.242 and 0.08±0.242 (mmol g-1), respectively, while the drop experienced on day 28 may be due to the utilization of available glucose by microorganisms for metabolic activities that led to biodegradation of crude oil. The amylase activity in the soil upon crude oil contamination reduced from 151.40±1.22 to 150.80±1.22 IU g-1. Statistical analysis shows that treatment of crude oil contaminated soil with sawdust and poultry manure increased soil amylase activity (p<0.05). The results of this study suggest that a combination of poultry manure and sawdust in bioremediation of crude oil contaminated soil is a solution to the problem of oil spillage in the Niger Delta region of Nigeria.