Abstract: It has been found through significant literature that there have been an increasing number of classroom learning environment research studies employing student perceptual data over the past 30 years however rarely has there been a classroom learning environment study in chemistry class in china and Jordan, so this study is very important to compare between the environment of the chemistry laboratories in china and Jordan, to compare the perceptions of learning environments by males and females in the chemistry laboratories at HZNU and JU by using the Science Laboratory Environment Inventory (SLEI). The SLEI have 5 scales for assessing student cohesiveness, open-endedness, Integration, Rule clarity and material environment. The sample consisted of 311males and 285 female. According to the study results significant difference were found between the study samples refer to the gender, experience and country.