Abstract: The present research demonstrates the biological activity of Dipterygium glaucum since extensive literature survey has shown no documented biological activity of this plant. Ethanol (80%) extract (A) of the plant was subfractioned by hexane (B), ethyl acetate (C) and butanol (D). These fractions (1 mg mL^-1) of D. glaucum showed 45 to 100% phytotoxicity as determined by the inhibition of Lemna minor plant growth but showed no cytotoxicity by brine shrimp lethality assay. DPPH radical scavenging activity of fraction `A` was 87% whilst other fractions had antioxidant activity below 35%. When fraction `A` was tested for antispasmodic activity, spontaneous contractions were recorded at 0.1-3.0 mg mL^-1 concentration in isolated rabbit jejunum preparations. It also inhibited K⁺ induced contractions to 60% at 1-3 mg mL^-1 level, suggesting a calcium channel blockade activity. The n-hexane (B), ethyl acetate (C) and n-butanol (D) fractions exhibited no or little antibacterial, antifungal, antileishmanial and insecticidal activities as compared with their respective controls. This is the first report on the biological activity of D. glaucum.