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Abstract: The present study is concerned with the production of amyloglucosidase by Aspergillus niger GCUCM-36. Effect of addition of different carbon sources and nitrogen sources on the production of enzyme was investigated. The enzyme formation was maximum (1180 IU/g/min) in the presence of glucose (1.0%) and NH4Cl (1.5% nitrogen). The production of enzyme reached maximum, (1180 IU/g/min) at 48 hours after incubation.