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Abstract: The shoots of Rauvolfia
serpentina from the field growing plants were used as explants and
cultured on MS medium supplemented with 2.5 mg/L BA + 0.1 mg/L NAA to
afford multiple shoots. It was evident that MS + 0.5 mg/L BA + 0.2 mg/L
NAA was the most suitable combination for induction of callus and development
of shoot buds formation but it failed to support elongation of shoot.
So, the cultures with tiny shoots was transferred to 2.0 mg/L BA with
0.05mg/L NAA, where they showed excellent elongation. Regenerated shoots
rooted well 0.2 mg/L IBA+ 0.2mg/L NAA combination. After having acclimatization
the regenerated plantlets were transferred to the open field where 95%
of the plant-lets survived and showed excellent growth.