Abstract: The present study was carried out to assess prevalence of hepatitis B virus (HBV) in relation to sex, age, promotive factors, associated symptoms and season among human urban population of Multan, Pakistan for a period of 32 months from June 2001-February 2004. The present study was based on the data, collected randomly from the human population aging from 1-72 years. The population was divided into three age groups i.e old (age above 50 years), mature (age 13-50 years) and young (age below 13 years). The results from the present study suggested that among the observed urban population of Multan (n=2531), prevalence of HBV was 7.94%. The prevalence of HBV when studied in different age groups of both sexes, it was found that prevalence of HBV was maximum (9.63%) in mature males as compared to young males (6.14%) and old males (5.44%). The prevalence of hepatitis B surface antigen was higher (7.94%) in young females as compared to mature females (7.32%) and old females (6.80%). The main promotive factors for HBV were unscreened blood transfusion 56 (27.86%), infected syringes 34 (16.91%), contaminated barber, parlor tools 32 (15.92%), contaminated dentist equipments 29 (14.43%) and contaminated surgery equipment 25 (12.44%). The reported cases of HBV positive patients were maximum in summer 146 (72.63%) as compared to 55 (27.36%) in winter.