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Abstract: According to the published sequence of 14 avian Pasteurella multocida genes required for survival and expression in vivo, the swine P. multocida infected models in septicemic mice were established and 14 pairs of primers were designed and synthesized to amplify the corresponding gene of swine P. multocida CVCC 432 strain. Successfully, two genes, pm0221 and pm1069 were identified as in vivo-expression gene using RT-PCR. Their nucleotide sequences were compared with those of avian P. multocida pm70 strain and the homology were 98.8 and 94.8%, respectively.