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Abstract: In this study, the effects of beta receptor blocker (carazolol) on the main detoxifying enzymes, Superoxide Dismutase (SOD) and Catalase (CAT) in sheeps were investigated. Two group (n = 6) were design. 1 mL ewe-1 of serum physiologic were administered i.m. to the control group (Group 1) and 0.5 mg ewe-1 of carazolol were injected i.m. to the treatment group (group 2). After treatment the blood samples were collected at 24, 48 and 72 h. No differences were observed in antioxidant enzyme values in sheep plasma. Also, no effect was seen on oxidative stress marker. Here, we show that carazolol may be alternatively used as a β-blocker agent in sheeps.