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Abstract: This experiment was conducted for investigation, the influence of prebiotic, garlic powder and turmeric powder on interior organs and hematological indices of broilers. Based a randomized completely design, 240 days old Ross 308 broilers were distributed into 16 floor pens and reared for 42 days. A basal diet was formulated according to NRC recommendations for starter (0-21 days) and grower (22-42 days) periods. The basal diet was also supplemented with prebiotic Biolex-MB, garlic powder and turmeric powder, resulting 4 dietary treatments were prepared including control group. Each dietary treatment was fed ad-libitum to 4 replicates group of 15 birds at the bigining of rearing period. The lowest (p<0.05) abdominal fat percent and serum cholesterol and triglycerides levels were recorded for broilers fed the diet supplemented with Biolex-MB and garlic powder, While, the highest High Density Lipoprotein (HDL) was recorded for birds fed diet supplemented with garlic powder. Inclusion of Biolex-MB and garlic powder into the diets significantly decreased concentration of Low Density Lipoprotein (LDL) and Very Low Density Lipoprotein (VLDL) in serum (p<0.05).