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Journal of Animal and Veterinary Advances
Year: 2008  |  Volume: 7  |  Issue: 4  |  Page No.: 409 - 412

Evaluating Antibacterial Activity of the Iranian Honey Through MIC Method on Some Dermal and Intestinal Pathogenic Bacteria

Mohammad Reza Dastouri, Kamran Fakhimzadeh, Jalal Shayeg, Jalil Dolgari-Sharaf, Mohammad Reza Valilou and Naser Maheri-Sis    

Abstract: In this study, the collected multi floral honey from bee colonies in province of East Azerbaijan (Iran) was prepared the doubling dilution and impregnated on paper disks. These disks were used on bacterial cultured media and their antibacterial effects were assessed by disk diffusion test method. The selected bacteria were provided through the standard strains, in addition to that some of the clinical samples were also selected and the taken subsequences were compared with the standard strains. The positive extracts in the disk diffusion method, were selected and used in MIC (Minimum Inhibitory Concentration) method on the specific bacteria. The MBC (Minimum Bactericidal Concentration) method as a complimentary test also was performed on them. In the above mentioned tests, control (untreated) disks were also considered. The results showed that the obtained extracts of Iranian multi floral honey (from the bee colonies of East Azerbaijan Province) have broad spectrum antibacterial activities, although their efficacy showed diversity on different bacterial species.

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