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Asian Journal of Animal and Veterinary Advances
Year: 2008  |  Volume: 3  |  Issue: 4  |  Page No.: 214 - 221

Use of Nylon Bag Technique to Determine Nutritive Value and Degradation Kinetics of Iranian Alfalfa Varieties

Afshar Mirzaei-Aghsaghali, Naser Maheri-sis, Ali Mirza-Aghazadeh, Amir Reza Safaei, Amir-Farhang Houshangi and Abolfazl Aghajanzadeh-Golshani    

Abstract: The aim of the study was to determine the chemical composition and ruminal degradation characteristics of two alfalfa varieties including Kareyonge (KAR) and Hamedani (HAM) grown in West Azerbaijan in Iran, using in situ technique. The nutritional parameters were Dry Matter (DM), Organic Matter (OM) and Crude Protein (CP). Nylon bags filled with 5 g of each forage were suspended in the rumen of three cannulated Gezel rams immediately before feeding and incubated for 7 different times (0, 4, 8, 16, 24, 48 and 72 h). Parameters for degradation kinetics included readily degraded fraction, slowly degraded fraction, lag time and fractional rate of passage. No significant difference found between DM, CP, Ash and Ether Extract (EE) of two alfalfa varieties although the difference for Crude Fiber (CF), Neutral Detergent Fiber (NDF) and Acid Detergent Fiber (ADF) were significant (p<0.01).The degradability of DM, OM, CP and Effective Rumen Degradable Protein (ERDP) at a rate of 0.05/h for KAR and HAM varieties were 54.2, 52.16, 40.1%, 72.57 g kg-1 DM and 56.97, 54.9, 39.25%, 89.4 g kg-1 DM, respectively. Calculations based on in situ degradability indicate that HAM alfalfa can have a higher inclusion than KAR alfalfa in diets for ruminants because of lower NDF, greater cell contents and ERDP.

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