Abstract: This study deals with isolation and identification of Pseudomonasaeruginosa from marine environment for phenazine pigment production. Pigment production results revealed that, the strain could able to produce two different pigments namely, pyocyanin and pyorubrin. Maximum biomass was observed at 66 h of incubation, but the pigment production seems to be continuous throughout the culture period (72 h). Antibacterial activity of the pigments was evaluated against pathogenic bacteria, maximum growth inhibitory activity was observed with pyocyanin and pyorubrin at 20 μL concentration (1.7 and 1.3 cm, respectively) against Citrobacter sp. Hemolytic activity of the pigments inferred that, hemolysis was observed with both pigments at 15, 20 and 25 μL concentration and no hemolysis was found at 5, 10 and 15 μL. The pigments were evaluated for their potential as food colourants with agar. Pleasant colouration was observed with pyocyanin and pyorubrin at 25 mg mLG-1 concentration.
08 February, 2011
amany salama el sharkawy: dear sir,
I'm a master student from egypt,biotechnology department,institute of graduate studies and research,alexandria,i succeeded to isolate marine pseudomonas aeruginosa producing pyocianin and pyorubrin,but i used pigment production media at 28 degree temprature a nd 48 hour incubation,i wish to know if you obtained the blue green pyocyanin at the same cultural conditions as to obtain the brown red pyorubrin???it was different in my case,,on the other hand ,,did you try acetone precipitation and coloum chromatography to purify the product??if you are willing to share information and discuss it with me please e mail .best of luck in your research.thanks,bye