The aim of this study was to formulate and optimize repaglinide (Rg) loaded chitosan (CN) nanoparticles as a sustained release. Repaglinide is a hypoglycemic agent of the meglitinide analog. In present study repaglinide loaded chitosan nanoparticles were prepared by solvent evaporation method in three different ratios. In this method weighed quantity of drug and polymer were dissolved in suitable organic solvent acetone and 2% acetic acid (organic phase). This solution was added drop by drop to the aqueous phase of PVA and homogenized using homogenizer at 18000 rpm followed by magnetic stirring for 2-3 h. The formed Rg-CN nanoparticles were recovered by centrifugation at 25,000 rpm for 15 min followed by washing thrice with petroleum ether and lyophilized. The prepared nanoparticles were evaluated for particle size, Scanning Electron Microscopy (SEM), Fourier Transform Infra Red spectroscopy study (FT-IR), percentage yield, drug entrapment and for in vitro release kinetics. Among the three different ratio 1:4 ratio shown high drug loading (11.22% w/w) and encapsulation efficiencies (97.0%) and nanoparticle recovery (86.40%) with nanosize. Scanning electron microscopy exposed that nanoparticles were spherical in shape with a nearly smooth surface morphology. Particle size was analyzed by Malvern particle size analyzer and shown 48-100 nm range. FT-IR study reveals that, there was no interaction between repaglinide and polymers. Based on the in vitrostudy, replaglinide released from prepared formulation was slow and sustained over 15 days. Application of the in vitro drug release data to various kinetic equations indicated first order release, swelling and diffusion mechanism from repaglinide nanoparticles.
G. Poovi, U.M. Dhana lekshmi, N. Narayanan and P. Neelakanta Reddy, 2011. Preparation and Characterization of Repaglinide Loaded Chitosan Polymeric Nanoparticles. Research Journal of Nanoscience and Nanotechnology, 1: 12-24.