Two variety of tomato viz. manik and pusha rubi were used to evaluate the efficacy of antagonistic P. fluorescens in controlling wilt of tomato caused by R. solanacearum and in increasing yield in green house condition. Four treatments i.e. R. solanacearum (106 c.f.u./ml) was used as control and P. fluorescens was used as 109, 108 and 107 c.f.u./ml suspension. Manik (V1) showed least bacterial wilt incidence and pusa rubi (V2) showed highest wilt incidence at 30, 45 and 60 DAI. T1 (P. fluorescens 109 c.f.u./ml) and combined effect of V1T1 decreased wilt of tomato at all stages and increased fruit yield. T0 (control-only R. solanacearum 106 c.f.u./ml) and V2T0 increased bacterial wilt at all stages and decreased fruit yield. So, T1 and V1T1 may be used for controlling bacterial wilt of tomato and increasing yield.