Abstract:
Random amplified polymorphic
DNA (RAPD) analysis was employed on twenty-four Streptococcus uberis
isolates from cases of clinical and sub-clinical mastitis from five
smallholder dairy herds in Malaysia. Three ten-mer primers namely OPA-01,
OPA-05 and OPA-07 were selected out of a 20 primers set. A high degree
of genetic polymorphism was revealed among the isolates of S. uberis
by RAPD-PCR. Fourteen distinct RAPD fingerprint patterns were generated
with primer OPA-05, while primers OPA-01 and OPA-07 produced 12 and
11 RAPD patterns respectively. The discriminatory power of this technique
could be further improved by the parallel use of several primers. Most
of the isolates were genetically different strains, however, identical
patterns were noted among different cows within the same farm or from
different cows from different farming regions. These findings indicate
that RAPD analysis is a rapid and reproducible method for molecular
sub-typing of bovine S. uberis isolates and therefore, represents
a powerful tool for epidemiological studies in comparison with the other fractions and its action was mainly due to the presence of linoleic acid in this fraction. Hydrocarbon and polar fractions did not show any significant activity as phytotoxic agents.
Umakanta Sarker , P. S. Biswas , B. Prasad and M. A. Khaleque Mian , 2002. Heterosis and Genetic Analysis in Rice Hybrids. Pakistan Journal of Biological Sciences, 5: 1-5.
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