The aim of study was to evaluate the antagonistic activities of bacteria associated with maize husks and cobs against some phytopathogenic and spoilage fungi namely: Fusarium oxysporum, F. solani, Colletotrichum lindemuthianum, Rhizoctonia solani, Phytophthora infestans, Aspergillus niger, A. flavus and Penicillium italicum. The streak bioassay method was employed in determining the antifungal capabilities of the isolated bacteria. Six bacteria were isolated from maize husks and were identified as Lactobacillus sp., Proteus sp., Sporolactobacillus sp., Clostridium sp., Micrococcus sp. and Klebsiella sp. while Lactobacillus sp., Providencia sp. and Bacillus subtiliswere isolated from maize cob. In vitro bioassay confirmed that only B. subtilis was antagonistic to Fusarium oxysporum, F. solani, Rhizoctonia solani Phytophthora infestans and Penicillium italicum but was not antagonistic to Aspergillus niger, A. flavus and Colletotrichum lindemuthianum,. B. subtilis has the greatest inhibitory effect on R. solani and least on F. oxysporum with percentage inhibition of 89 and 50, respectively on the fifth day. The B. subtilis was fungistatic in action. The inhibitory effect of Bacillus subtiliswas attributed to the production of diffusible antifungal compounds.