In the present study Colletotrichum lindemuthianum was isolated from leaves of infected cowpea (variety IT 90K-76) plants showing characteristic anthracnose symptoms. The efficacy of 12 different concentrations of ethanolic extract of bee-propolis prepared by serial dilution with distilled water from crude extract and incorporated into Saboraud dextrose agar plus acetic acid medium on the mycelial growth of Colletotrichum lindemuthianum (in vitro) was studied. Infra-red analysis of the propolis extract was carried out using Ultra-violet photospectrometer. Data obtained were subjected to analysis of variance (ANOVA) and treatment means were separated using Duncans New Multiple Range Test. Fungitoxicity of the propolis extract concentrations were expressed as percentage inhibition of mycelia growth. Mycelial growth was not significantly (p≤0.05) reduced in media containing propolis extract of 1-5%. Media containing propolis extract of 6-10% inhibited mycelial growth for 2, 3, 17, 18 and 21 days respectively and also significantly (p≤0.05) reduced mycelial growth. Media containing propolis extract of 20 and 30% proved fungicidal to C. lindemuthianum. Infra-red analysis revealed the likely presence of phenolics in the propolis extract.
K.C. Obasa , A.Y.A. Adeoti , O.A. Enikuomehin and J.G. Bodunde , 2007. Efficacy of Bee-Propolis in the Control of Colletotrichum lindemuthianum (Sacc. and Magn.) Briosi and Cav. in vitro
. Research Journal of Microbiology, 2: 175-179.