In the attempt of understanding the importance of the first generations in wheat breeding, a controversial experimentation procedure was used with F2 generation preceding F1. Ten bread wheat F2s and their parents were evaluated in dense stand followed by the evaluation of the ten F1s and their parents in isolation environment for two successive years. Three criteria groups were used; a) the heterosis of F1 and F2 according to their parents, b) the productivity and stability per se of F1 and F2 and c) the general/specific combining ability of parents. Heterosis alone proved of little significance in connecting the performance of both generations compared to standard check heterosis. The highest yielding F2 was the only cross exhibiting negative heterosis and heterobeltiosis in F1 while it was significantly higher in F2 and equal in F1 for standard check heterosis constituting it a safe criterion. The second criteria group indicated significant correlation between the stability of F1 and F2 with the productivity of the crosses in total being equal to each other giving another linkage factor between the two generations. The diallel analysis of both experiments pinpointed the importance of the information provided by the F2 generation thus constituting it far more valuable than the information of F1. The data indicated that non heterotic F1s should not be discarded as a combined use of all the criteria can evaluate and discriminate more accurately the promising materials.
Charalampos A. Gogas and Metaxia Koutsika-Sotiriou, 2012. Phenotyping and Genotyping through F1 and F2 Generation the Promising Crosses. International Journal of Plant Breeding and Genetics, 6: 217-227.