The present study was directed to the production of N-acetyl-D-glucosamine (GlcNAc) by partial hydrolysis of colloidal α-chitin using the crude enzyme preparation of Aeromonas sp. PTCC 1691. The selective production of GlcNAc was observed during continuous hydrolysis of chitin and at the same time period, the production of GlcNAc oligomers was negligible (below 0.5%). The maximum production of GlcNAc, a yield of 79%, was obtained after 24 h of incubation at the enzyme-substrate ratio of 2 U mg-1. These results suggest that the multi-chitinolytic enzyme complex produced by Aeromonas sp. PTCC 1691 could be useful for bioconversion of chitin waste into N-acetyl-D-glucosamine for industrial applications.
K. Jamialahmadi, J. Behravan, M. Fathi Najafi, M. Tabatabaei Yazdi, A.R. Shahverdi and M.A. Faramarzi, 2011. Enzymatic Production of N-Acetyl-D-Glucosamine from Chitin Using Crude Enzyme Preparation of Aeromonas sp. PTCC1691. Biotechnology, 10: 292-297.